火焰兰等5种兰花菌根显微结构及分离培养基筛选  

Endomycorrhizal Structure and Solid Medium Selection of Five Orchid Species including Renanthera coccinea Lour.

作　　者：钟国和 黄赛 云勇 黎肇家 任军方 Zhong Guohe;Huang Sai;Yun Yong;Li Zhaojia;Ren Junfang(Hainan Academy of Forestry(Hainan Academy of Mangrove),Haikou Hainan 571100;Institute of Tropical Horticulture,Hainan Academy of Agricultural Sciences,Haikou Hainan 571100;Sanya Research Institute,Hainan Academy of Agricultural Sciences,Sanya Hainan 572000)

机构地区：[1]海南省林业科学研究院(海南省红树林研究院),海南海口571100 [2]海南省农业科学院热带园艺研究所,海南海口570100 [3]海南省农业科学院三亚研究院,海南三亚572025

出　　处：《热带林业》2024年第1期56-59,共4页Tropical Forestry

基　　金：2013年院科技创新专项(2013-15):海南两种珍稀濒危野生兰花菌根真菌研究;2019年院二类研究所辅助项目(2019-2-25):墨兰组织培养及菌根真菌的研究;2020年院预算项目(农业科技创新专项)(KJCX-2020-08):海南岛火焰兰属优异种质筛选及鉴定。

摘　　要：通过石蜡切片法对5种不同类型的兰花新鲜营养根的显微结构进行研究,并利用组织分离法对其中的内生真菌进行分离,从而筛选最佳的分离培养基。结果表明:5种兰花菌根结构一致,由外到内依次为根被、皮层、中柱,三个部位都有不同程度的被真菌侵染表现,海南钻喙兰、火焰兰是通过皮层细胞直接侵染,卷萼兜兰、硬叶兜兰和铁皮石斛是通过根毛或皮层细胞侵染,并形成菌丝团。实验结果显示:PDA培养基最适合做5种兰花菌根分离纯化培养基。Using five orchid species as experimental materials,Observing the microstructure of mycorrhizal fungi using paraffin sectioning method.And using tissue isolation method to isolate and culture endophytic fungi,for selecting the optimal culture medium.The results showed that the root structures of the five orchids are root cover,cortex and stele which all infected with mycorrhizal fungi to varying degrees.Epiphytic orchids are directly infected by cortical cells.Saprophytic orchids are infected by root hairs or cortical cells,and form a mycelium mass.PDA medium is the most suitable medium for isolation and purification of orchid mycorrhizal fungi.

关 键 词：5种兰花 菌根结构 培养基筛选 

分 类 号：Q949.718.43[生物学—植物学]