白细胞介素22及其受体1基因多态性与海南汉族人群支气管哮喘易感性的关系  

Relationship between interleukin-22 and interleukin-22 receptor 1 gene polymorphism and bronchial asthma susceptibility in Hainan Han population

作　　者：钟培雄 陈钰 郝金香 吴挺实 黄一桂 Zhong Peixiong;Chen Yu;Hao Jinxiang;Wu Tingshi;Huang Yigui(Department of Respiratory,the Third People's Hospital of Haikou,Haikou 571100,China)

机构地区：[1]海口市第三人民医院呼吸内科,海口571100

出　　处：《国际流行病学传染病学杂志》2024年第1期43-48,共6页International Journal of Epidemiology and Infectious Disease

摘　　要：目的探讨IL-22及其受体1基因多态性与海南汉族人群支气管哮喘易感性的关系。方法选取2019年1月至2022年10月海口市第三人民医院收治的135例哮喘患者作为哮喘组,正常者85例作为对照组。比较两组人群血清IL-22、IL-22受体1和IgE水平,分析IL-22和IL-22受体1基因各位点的基因型和等位基因频率分布情况。应用Logistic回归分析IL-22和IL-22受体1基因多态性与海南汉族人群哮喘发病的关系。结果哮喘组血清IL-22[(61.37±12.40)pg/mLvs(26.25±8.72)pg/mL]、IL-22受体1[(46.28±10.51)pg/mLvs(14.50±4.63)pg/mL]及IgE[(304.72±116.37)IU/mLvs(59.36±9.80)IU/mL]水平均明显高于对照组(t=16.22、18.60和12.41,均P<0.001)。哮喘组rs2227485位点GG(42.2%vs 20.0%)、GA基因型(34.1%vs 63.5%),rs1179251位点CC(61.5%vs 41.2%)、CT基因型(29.6%vs 50.6%)和rs3795299位点CC(57.0%vs 41.2%)、CG基因型(30.4%vs 45.9%)分布频率与对照组比较,差异均有统计学意义(χ^(2)=11.54、8.65和5.42,P=0.001、0.003和0.020)。rs2227485位点GA基因型、rs1179251位点CT基因型、rs3795299位点CG基因型患者的血清IL-22水平[(42.58±9.37)pg/mL、(77.25±22.36)pg/mL和(68.12±15.48)pg/mL],IL-22受体1水平[(31.15±7.20)pg/mL、(57.14±17.20)pg/mL和(52.36±15.28)pg/mL]及IgE[(125.73±50.36)IU/mL、(412.37±185.62)IU/mL和(381.26±151.20)IU/mL)]水平明显低于其他基因型(F=15.71、13.93、16.81、14.61、11.85、17.23、14.99、15.11和13.92,均P<0.001)。Logistic回归分析显示,rs2227485位点GA基因型(OR=0.893,95%CI:0.712~0.954)、rs1179251位点CT基因型(OR=0.794,95%CI:0.591~0.826)及rs3795299位点CG基因型(OR=0.840,95%CI:0.668~0.913)与哮喘发病风险降低有关。结论IL-22及IL-22受体1基因多态性可能与海南汉族人群哮喘易感性有关,其中GA、CT和CG基因型能够降低哮喘发病风险。Objective To investigate the relationship between interleukin-22(IL-22)and IL-22 receptor 1(IL-22R1)gene polymorphisms and the susceptibility to bronchial asthma in Hainan Han population.Methods A total of 135 patients with asthma in the Third People's Hospital of Haikou from January 2019 to October 2022 were selected as asthma group,and 85 normal individuals were selected as the control group.The serum levels of IL-22,IL-22R1 and IgE were compared between the two groups,and the genotype and allele frequency distribution of IL-22 and IL-22R1 genes were analyzed.Logistic regression analysis was used to determine the relationship between IL-22 and IL-22R1 gene polymorphisms and the incidence of asthma in Hainan Han population.Results The levels of serum IL-22[(61.37±12.40)pg/mL vs(26.25±8.72)pg/mL],IL-22R1[(46.28±10.51)pg/mL vs(14.50±4.63)pg/mL]and IgE[(304.72±116.37)IU/mL vs(59.36±9.80)IU/mL]in asthma group were significantly higher than those in the control group(t=16.22,18.60 and 12.41,all P<0.001).The distribution frequencies of GG(42.2%vs 20.0%)and GA genotype(34.1%vs 63.5%)at rs2227485,CC(61.5%vs 41.2%)and CT genotype(29.6%vs 50.6%)at rs1179251,and CC(57.0%vs 41.2%)and CG genotype(30.4%vs 45.9%)at rs3795299 in asthma group were significantly different from those in the control group(χ^(2)=11.54,8.65 and 5.42,P=0.001,0.003 and 0.020).The levels of serum IL-22[(42.58±9.37)pg/mL,(77.25±22.36)pg/mL and(68.12±15.48)pg/mL],IL-22R1[(31.15±7.20)pg/mL,(57.14±17.20)pg/mL and(52.36±15.28)pg/mL]and IgE[(125.73±50.36)IU/mL,(412.37±185.62)IU/mL and(381.26±151.20)IU/mL]in the GA genotype at rs2227485,CT genotype at rs1179251 and CG genotype at rs3795299 were significantly lower than those in other genotypes(F=15.71,13.93,16.81,14.61,11.85,17.23,14.99,15.11 and 13.92,all P<0.001).Logistic regression analysis showed that the GA genotype of rs2227485(OR=0.893,95%CI:0.712-0.954),the CT genotype of rs1179251(OR=0.794,95%CI:0.591-0.826)and the CG genotype of rs3795299(OR=0.840,95%CI:0.668-0.913)were associated wit

关 键 词：哮喘 白细胞介素-22 白细胞介素-22受体1 基因多态性 易感性 

分 类 号：R562.25[医药卫生—呼吸系统]