两个家蚕CPFL表皮蛋白基因的鉴定与表达分析  

Identification and Expression Analysis of Two CPFL Genes in Silkworm(Bombyx mori)

作　　者：刘惠芬[1] 华丽峰[1] 刘文光[1] 娄齐年[1] 衣葵花[1] 李云芝[1] 郭光[1] Liu Huifen;Hua Lifeng;Liu Wenguang;Lou Qinian;Yi Kuihua;Li Yunzhi;Guo Guang(Shandong Sericultural Research Institute,Yantai 264002,China)

机构地区：[1]山东省蚕业研究所,山东烟台264002

出　　处：《山东农业科学》2024年第3期139-144,共6页Shandong Agricultural Sciences

基　　金：山东省蚕桑产业技术体系建设项目(SDAIT-18-02,SDAIT-18-03);山东省农业科学院农业科技创新工程项目(CXGC2023C03);国家现代农业产业技术体系烟台综合试验站项目(CARS-18-SYZ08)。

摘　　要：昆虫CPFL表皮蛋白是一类重要的结构蛋白。本研究通过生物信息学分析从家蚕基因组中鉴定出2个CPFL基因,命名为BmHCP1和BmHCP2。氨基酸序列分析发现,2个蛋白在羧基末端均具有CPFL表皮蛋白典型的保守结构序列YGW以及重复序列AAH、AAPA、AAPV。进化树分析显示,2个蛋白与鳞翅目昆虫艺神袖蝶(Heliconius erato)和柑橘凤蝶(Papilio xuthus)具有较高同源性。荧光定量PCR分析显示,BmHCP1和BmHCP2存在组织、时相特异性表达,幼虫期在头部、中肠、表皮等组织中表达量较高;蛹期除翅原基、血淋巴、触角和足等组织外均有较高表达量;成虫期在足、触角、翅原基、表皮等组织中表达量较高;蛹期在大多数组织的表达量明显高于幼虫期和成虫期。研究结果为进一步探明家蚕表皮蛋白基因的功能奠定了基础。CPFL cuticular proteins of insect are a kind of important structural proteins.Two CPFL genes designated as BmHCP1 and BmHCP2 were identified from the silkworm genome using bioinformatics method in this study.The amino acid sequence alignment indicated that both the two proteins contained the conserved structural sequences YGW and repetitive sequences AAH,AAPA and AAPV of CPFL proteins at the carboxyl terminal.The phylogenetic tree analysis showed that the two CPFL proteins had high homology with lepidoptera insect Heliconius erato and Papilio xuthus.Real-time PCR detection results indicated that BmHCP1 and BmH-CP2 were diversely expressed in different tissues.Their expression levels were higher in head,midgut and cu-ticular during silkworm larval phase;except wing primordium,hemolymph,antennae and feet,higher expres-sion was observed in all tissues during pupal stage;their expression levels were higher in wing primordium,epidermis,feet and antennae of silkworm moth.The expression levels of the two CPFL genes in most tissues were significantly higher at pupal stage than those at larval and adult stages.These results could lay a founda-tion for further exploring the function of the cuticular proteins in silkworm.

关 键 词：家蚕 CPFL表皮蛋白 序列分析 表达分析 荧光定量PCR 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q781[农业科学—畜牧兽医]