柠檬汁还原法制备AgNPs用于果蔬农药残留的SERS快速检测  

AgNPs Prepared by Lemon Juice Reduction Method for SERS Rapid Detection of Pesticide Residues in Fruits and Vegetables

作　　者：董闪闪[1,2,3] 张凤秋 夏琦 李佳林 刘超 柳少伟 陈翔宇[2,3] 王儒敬 黄青 DONG Shanshan;ZHANG Fengqiu;XIA Qi;LI Jiain;LIU Chao;LIU Shaowei;CHEN Xiangyu;WANG Rujing;HUANG Qing(School of Physics and Laboratory of Zhongyuan Light,Zhengzhou University,Zhengzhou 450001,China;Agricultural Sen‐sors and Intelligent Perception Technology Innovation Center of Anhui Province,Zhongke Hefei Institutes of Collaborative Research and Innovation for Intelligent Agriculture,Anhui Hefei 231131,China;Intelligent Agriculture Engineering Laboratory of Anhui Province,Institute of Intelligent Machines,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Anhui Hefei 230031,China;School of Materials and Chemistry,Anhui Agricultural University,Hefei 230036,China;Institute of Physical Science and Information Technology,Anhui University,Hefei 230601,China)

机构地区：[1]郑州大学物理学院、中原之光实验室,河南郑州450001 [2]中科合肥智慧农业协同创新研究院,农业传感器与智能感知安徽省技术创新中心,安徽合肥231131 [3]中国科学院合肥物质科学研究院,智能机械研究所,安徽省智慧农业工程实验室,安徽合肥230031 [4]安徽农业大学材料与化学学院,安徽合肥230036 [5]安徽大学物质科学与信息技术研究院,安徽合肥230601

出　　处：《智慧农业（中英文）》2024年第1期101-110,共10页Smart Agriculture

基　　金：中国科学院合肥研究院长融合基金项目(E02AAG85135)。

摘　　要：[目的/意义]为满足目前市场上对农产品农药残留的快速灵敏检测需求,报道一种基于柠檬汁还原制备银纳米粒子(AgNPs)的方法。[方法]首先将新鲜柠檬汁经滤纸过滤,稀释成2%的柠檬汁水溶液,再配制一定浓度的AgNO3溶液、50 mM的NaOH溶液,放置室温保存。然后在室温下,将10 mL的ddH2O、2mL的NaOH、2mL的2%柠檬汁和5 mL的AgNO3溶液混合,待溶液颜色变为澄清的黄色时,溶液离心即可获得AgNPs。[结果和讨论]该方法制备的AgNPs,其颗粒形貌大小基本均一,约为20 nm,具有很好的表面增强拉曼散射(Surface Enhancement of Raman Scattering,SERS)增强效应,即良好的SERS信号稳定性,较强的SERS增强性能。该胶体中AgNPs分散较均匀,并且具有较长时间储存的稳定性,因此可用于微量农残检测。柠檬汁中主要还原成分抗坏血酸、葡萄糖和果糖,其含量分别为395.76μg/mL、5.95 mg/mL和5.90 mg/mL。将柠檬汁还原法制备的AgNPs用于果蔬表面农残检测,对于百草枯、多菌灵的检出限分别最低至3.90 ng/kg及0.22μg/kg。[结论]这项工作为果蔬农残快检提供了一种绿色、便捷的SERS材料制备方法,为实现农产品农药残留的快速、灵敏检测提供一种新的途径。[Objective]The use of pesticides is one of the root causes of food safety problems.Pesticide exposure and pesticide residues can not only lead to environmental pollution issues but also seriously affect human health.In order to meet the rapid and sensitive detection needs of pesticide residues in agricultural products,a method based on lemon juice reduction to prepare silver nanoparticles(AgNPs)is reported in this research.[Methods]First,fresh lemon juice was filtered through filter paper and diluted to a 2%lemon juice aqueous solution.Then,a certain concentration of AgNO3 solution,50 mm NaOH solution were prepared and stored at room temperature.Then,10 mL ddH2O,2 mL NaOH,2 mL 2%lemon juice,and 5 mL AgNO3 solution were mixed.When the solution turned to a clear yellow color,the solution was centrifuged to obtain AgNPs.The morphology and structure of AgNPs were observed by transmission electron microscopy(TEM).In order to verify the successful synthesis of the nanoparticles and the distribution characteristics of the nanoparticles,ultravio‐let spectroscopy was used for measurement and analysis,and 4-ATP was used as a SERS probe to preliminarily verify the SERS en‐hancement performance of AgNPs.Furthermore,the content of the main reducing components in lemon juice,namely ascorbic acid,glucose,and fructose was analyzed.The content of ascorbic acid in lemon juice was determined by high-performance liquid chroma‐tography,and the content of glucose and fructose in lemon juice was determined by UV-visible spectrophotometry.To verify the stabil‐ity and uniformity of the SERS signal of the nanoparticles,4-ATP was used as an surface enhancement of raman scattering(SERS)probe for detection analysis.The stability of the SERS performance of the colloidal substrate within 41 days and the SERS perfor‐mance at temperatures ranging from 0-80°C were analyzed.Using 4-ATP as the SERS probe,the experimental conditions were opti‐mized for the preparation of AgNPs by the lemon juice method,including pH and AgNO3 concentrati

关 键 词：表面增强拉曼散射/光谱(SERS) 银纳米粒子(AgNPs) 农药残留 果蔬 光谱检测 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]