BD-77雾化吸入给药对肺炎支原体感染小鼠的治疗作用  

Therapeutic effect of aerosol inhalation of BD-77 against mycoplasma pneumoniae infection in mice

作　　者：孙静[1] 赵荣华[1] 郭姗姗[1] 高双荣[1] 包蕾[1] 耿子涵 李舒冉 孙绮悦 徐洲 覃广源 潘玉杰 谭秋霞 龙忠义 黄成钢 崔晓兰[1] SUN Jing;ZHAO Ronghua;GUO Shanshan;GAO Shuangrong;BAO Lei;GENG Zihan;LI Shuran;SUN Qiyue;XU Zhou;QIN Guangyuan;PAN Yujie;TAN Qiuxia;LONG Zhongyi;HUANG Chenggang;CUI Xiaolan(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China;Guizhou Bailing Enterprise Group Pharmaceutical Co.,LTD.,Anshun Guizhou 561099,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国科学院上海药物研究所,上海201203 [3]贵州百灵企业集团制药股份有限公司,贵州安顺561099

出　　处：《中国药物警戒》2024年第3期253-256,262,共5页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金资助项目(82141206)。

摘　　要：目的评价BD-77雾化吸入给药对肺炎支原体肺炎小鼠模型的治疗作用。方法Balb/c小鼠按照体质量随机分为正常对照组、模型对照组、阿奇霉素对照组(42 mg·kg^(-1)·d^(-1))、BD-77高剂量组(75 mg·mL^(-1),雾化15 min)、BD-77低剂量组(37.5 mg·mL^(-1),雾化15 min)。以肺炎支原体滴鼻感染建立小鼠支原体肺炎模型,雾化给药4 d后,通过小鼠体重及肺重计算肺指数和肺指数抑制率,酶联免疫吸附试验(ELISA)检测肺组织中白介素-6(IL-6)、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)含量及血清中C反应蛋白(CRP)水平,苏木精-伊红(HE)染色评估小鼠肺组织病理学变化,全面评价BD-77对小鼠支原体肺炎的治疗作用。结果BD-772个剂量组雾化吸入给药15 min均可显著降低小鼠肺指数,减轻肺部炎症病变,降低肺组织中IL-6、IL-1β、TNF-α含量及血清CRP水平。结论BD-77雾化吸入可治疗支原体感染小鼠肺炎,本研究为BD-77开发作为防治肺炎支原体肺炎的药物提供了实验室数据支持。Objective To evaluate the therapeutic effect of aerosol inhalation of BD-77 against mycoplasma pneumoniae(MP)infection in mice.Methods Balb/c mice were randomly divided into the normal control group,model control group,azithromycin control group(42 mg·kg^(-1)·d^(-1)),BD-77 high dose group(75 mg·mL^(-1),15 min),and BD-77 low dose group(37.5 mg·mL^(-1),15 min).The mouse model of mycoplasma pneumoniae pneumonia(MPP)was induced by intranasal infection with MP.After four consecutive days of aerosol inhalation,the therapeutic effect of BD-77 was evaluated based on the lung index,lung inhibition rate,and pathological changes of lung tissues.The contents of interleukin(IL)-6,IL-1β,tumor necrosis factorα(TNF-α)in lung tissues and the level of C reactive protein(CRP)in serum were also detected using ELISA method.Results Both doses of BD-77 aerosol administration could decrease the lung index and the release of IL-6,IL-1βand TNF-αin lung tissues,ameliorate pulmonary inflammation and reduce serum CRP levels.Conclusion Aerosol inhalation of BD-77 preparation is effective for MPP in mice.This study provides data for the development of BD-77 as a drug for prevention and treatment of MPP.

关 键 词：BD-77 肺炎支原体 雾化吸入 炎症因子 肺指数 肺指数抑制率 小鼠 

分 类 号：R978[医药卫生—药品]