白藜芦醇在小鼠溃疡性结肠炎相关结直肠癌中发挥的作用  

Role of resveratrol in ulcerative colitis-related colorectal cancer in mice

作　　者：孙娟[1] 闻静[1] 王启之[1] 郑兆炜[1] 张成斌[1] 邵明[1] Sun Juan;Wen Jing;Wang Qizhi;Zheng Zhaowei;Zhang Chengbin;Shao Ming(Department of Gastroenterology,the Second Affiliated Hospital of Bengbu Medical University,Bengbu,Anhui 233000,China)

机构地区：[1]蚌埠医学院第二附属医院消化内科,安徽蚌埠233000

出　　处：《齐齐哈尔医学院学报》2024年第4期314-317,共4页Journal of Qiqihar Medical University

基　　金：2022年度蚌埠医学院自然科学重点项目(2022byzd086)。

摘　　要：目的 探讨白藜芦醇在小鼠溃疡性结肠炎相关结直肠癌中发挥的作用和可能的分子机制。方法 将30只雄性C57BL/6小鼠随机分为对照组和观察组两组,每组各15只。用氧化偶氮甲烷/葡聚糖硫酸钠(AOM/DSS)建立小鼠UC结肠癌模型。观察组小鼠通过管饲法接受150 mg/kg白藜芦醇,1次/d。对照组同步用等量的无菌生理盐水灌胃。第71天处死小鼠,观察两组小鼠结肠组织病理形态,分析比较两组小鼠结肠组织中长非编码RNA H19(lncRNA H19)、Bcl-2、生存素(Survivin)和信号传导转录激活因子3(STAT3)mRNA的表达情况。结果 观察组小鼠结肠组织中lncRNA H19、Bcl-2、Survivin和STAT3 mRNA表达水平低于对照组,差异有统计学意义(P<0.05)。观察组小鼠结肠组织中Survivin和pSTAT3蛋白表达水平低于对照组,且差异有统计学意义(P<0.05)。结论 在CAC小鼠发病过程中,白藜芦醇可以抑制UC小鼠肿瘤的生成,其作用机制可能与抑制Bcl-2、Survivin和STAT3的表达有关。Objective To explore the role of resveratrol in ulcerative colitis-related colorectal cancer in mice and possible molecular mechanism.Methods Thirty male C57BL/6 mice were randomly divided into the control group(15 mice) and the observation group(15 mice).Mouse UC colon cancer model was established by using azomethane oxide/dextran sodium sulfate(AOM/DSS).Mice in the observation group received 150 mg/kg resveratrol once a day by tube feeding.The control group was given the same amount of sterile saline simultaneously.The mice were killed on the 71th day,and the pathological morphology of the colon tissues of the two groups were observed.The expressions of long non-coding RNA H19(lncRNA H19),Bcl-2,Survivin and signal transduction and activator of transcription 3(STAT3) mRNA in the colon tissues of the two groups were analyzed and compared.Results The expression levels of lncRNA H19,Bcl-2,Survivin and STAT3 mRNA in the colon tissue of mice in the observation group were lower than those in the control group,and the differences were statistically significant(P<0.05).The expression levels of Survivin and pSTAT3 protein in the colon tissue of mice in the observation group were lower than those in the control group,and the differences were statistically significant(P<0.05).Conclusions Resveratrol can inhibit tumor formation in UC mice during the pathogenesis of CAC mice,and its mechanism may be related to inhibiting the expression of Bcl-2,Survivin and STAT3.

关 键 词：白藜芦醇 溃疡性结肠炎 结直肠癌 BCL-2 生存素 信号传导和转录激活因子3 

分 类 号：R285[医药卫生—中药学]