1-磷酸鞘氨醇受体3对脂多糖处理大鼠肾小管上皮细胞凋亡的影响  

Effect of S1PR3 on apoptosis of renal tubular epithelial cells treated with lipopolysaccharide

作　　者：吴晋[1] 应静 WU Jin;YING Jing(The First Affiliated Hospital of Ningbo University,Ningbo 315010,Zhejiang,China)

机构地区：[1]宁波大学附属第一医院,宁波315010

出　　处：《现代实用医学》2024年第2期166-169,共4页Modern Practical Medicine

基　　金：浙江省医药卫生科技计划项目(2020KY250);宁波市科技项目(2017A610192)。

摘　　要：目的探讨1-磷酸鞘氨醇受体3(S1PR3)对脂多糖(LPS)处理大鼠肾小管上皮细胞凋亡的影响。方法将培养好的大鼠肾小管细胞(NRK-52E)分为对照组、LPS组、S1PR3激动剂+LPS组。对照组不做处理;LPS组予LPS(20μg/ml)刺激12h后做离心收集待检;S1PR3激动剂+LPS组先用S1PR3激动剂KRX-725预处理2 h后再予LPS(20μg/ml)刺激12 h后做离心收集待检。流式细胞术检测各组细胞凋亡率、钙离子表达及caspase-3表达的差异,ELISA试剂盒检测Calpain 1、Calpain 2的表达差异,Western Blot检测各组细胞中S1PR3的表达差异。结果LPS处理后脓毒症NRK-52E细胞模型成功建立,相比于对照组,LPS组和S1PR3激动剂+LPS组的细胞凋亡率、S1PR3表达、钙离子浓度、Calpain 1与Calpain2的表达、caspase-3表达均显著增加(均P<0.05);与LPS组相比,S1PR3激动剂+LPS组的细胞凋亡率、钙离子浓度、Calpain 1与Calpain2的表达、caspase-3表达均显著增加(均P<0.05),S1PR3表达显著增加(P<0.05)。结论S1PR3的激活能够增加肾小管上皮细胞内的钙离子浓度,从而激活caspase-3细胞凋亡信号通路,导致脓毒症肾小管上皮细胞凋亡增加。Objective To investigate the effect of sphingosine 1-phosphate receptor 3(S1PR3)on apoptosis of re-nal tubular epithelial cells treated with lipopolysaccharide(LPS).Methods The cultured rat renal tubular cells(NRK-52E)were divided into control group,LPS group and S1PR3 agonist+LPS group.The control group was not treated;LPS group was stimulated by LPS(20 g/ml)for 12 h and centrifuged.S1PR3 agonist+LPS group was pret-reated with S1PR3 agonist KRX-725 for 2 h and then stimulated with LPS(20 g/ml)for 12 h.Flow cytometry was used to detect the differences in cell apoptosis rate,calcium ion expression and caspase-3 expression among all groups.ELISA kit was used to detect the differences in the expression of Calpain 1 and Calpain 2.Western Blot was used to detect the differences in the expression of S1PR3 among all groups.Results The NRK-52E cell model of sepsis was successfully established after LPS treatment.Apoptosis rate,S1PR3 expression,calcium ion concentration,Calpain 1,Calpain 2 expression and caspase-3 expression were significantly increased in LPS group and S1PR3 agonist+LPS group(all<0.05).Compared with LPS group,apoptosis rate,calcium ion concentration,Calpain 1,Calpain 2 ex-pression and caspase-3 expression in S1PR3 agonist+LPS group were significantly increased(all<0.05),and S1PR3 expression was significantly increased(<0.05).Conclusions The activation of S1PR3 can increase the concentration of calcium ions in renal tubular epithelial cells,and thus activate the apoptosis signaling pathway of cas-pase-3 cells,leading to the increase of apoptosis of renal tubular epithelial cells in sepsis.

关 键 词：脓毒症 1-磷酸鞘氨醇受体3 肾小管上皮细胞 凋亡 

分 类 号：R631[医药卫生—外科学]