Toll样受体5在类风湿关节炎患者外周血单核细胞中的表达及意义  

Expression and significance of Toll-like receptor 5 in peripheral blood mononuclear cells of patients with rheumatoid arthritis

作　　者：张雯鑫 熊金河[2] ZHANG Wenxin;XIONG Jinhe(School of Medical and Life Sciences,Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan 610075,China;不详)

机构地区：[1]成都中医药大学医学与生命科学学院,四川成都610075 [2]遂宁市中心医院风湿免疫科,四川遂宁629000

出　　处：《中国临床研究》2024年第3期379-382,共4页Chinese Journal of Clinical Research

基　　金：四川省卫生和计划生育委员会重点研究项目(16ZD049)。

摘　　要：目的 检测类风湿关节炎(RA)患者外周血单核细胞表面Toll样受体5(TLR5)表达水平,探讨其临床意义。方法 选取遂宁市中心医院2017年1月至2022年8月确诊为RA的患者140例,以及健康对照人群70例(正常对照组),RA患者依据病情活动评分红细胞沉降率(DAS28-ESR)分为高疾病活动组(n=70)以及中低疾病活动组(n=70),采用流式细胞术检测3组外周血单核细胞表面TLR5的阳性率,采用ROC曲线分析单核细胞TLR5表达对RA的预测价值。结果 正常对照组、中低活动组和高活动组单核细胞表面TLR5阳性率分别为(46.99±5.79)%、(50.80±5.96)%和(58.86±6.20)%,3组比较差异有统计学意义(F=71.550,P<0.01)。ROC曲线分析显示,TLR5诊断RA的ROC曲线下面积(AUC)为0.79,最佳截断值为49.50%,敏感性为72.86%,特异性为73.57%。结论 外周血单核细胞表达的TLR5参与RA发病和维持疾病活动,对RA有一定的预测价值。Objective To examine the expression levels of Toll-like receptor 5(TLR5) on peripheral blood monocytes in patients with rheumatoid arthritis(RA),and to explore its clinical significance.Methods A total of 140 RA patients,along with 70 healthy controls(normal control group) received in Suining Central Hospital from January 2017 to August 2022 were selected.The RA patients were divided into high disease activity group(n=70) and moderate-to-low disease activity group(n=70) based on the disease activity score 28-erythrocyte sedimentation rate(DAS28-ESR).Flow cytometry was used to determine the positive rate of TLR5 on peripheral blood monocytes among three groups.The predictive value of TLR5 expression on monocytes surface for RA was analyzed using receiver operating characteristic(ROC) curves.Results The positive rates of TLR5 on the surface of monocytes in normal control group,moderate-to-low disease activity group and high disease activity group were(46.99±5.79)%,(50.80±5.96)% and(58.86±6.20)%,respectively,and there was a statistically significant difference among the three groups(F=71.550,P<0.01).The area under the ROC curve for TLR5 in diagnosing RA was 0.79,with a cut-off value of 49.50%,sensitivity of 72.86%,and specificity of 73.57%.Conclusion The TLR5 expressed on peripheral blood monocytes is involved in the pathogenesis and maintenance of disease activity and has predictive value for RA.

关 键 词：TOLL样受体 类风湿关节炎 单核细胞 疾病活动 流式细胞术 

分 类 号：R593.22[医药卫生—内科学]