落叶松八齿小蠹伴生长喙壳真菌致病性研究  

Pathogenicity of Ophiostomatoid Fungi Associated with Ips subelongatus

作　　者：刘亚 周勤政 王慧敏 王正 吕全[1] LIU Ya;ZHOU Qin-zheng;WANG Hui-min;WANG Zheng;LU Quan(Key Laboratory of Forest Protection of National Forestry and Grassland Administration,Ecology and Nature Conservation Institute,Chinese Academy of Forestry,Beijing 100091,China;College of Plant Protection,China Agricultural University,Beijing 100193,China;College of Plant Protection,Shandong Agricultural University,Tai’an 271018,Shandong,China)

机构地区：[1]国家林业和草原局森林保护学重点实验室中国林业科学研究院森林生态环境与自然保护研究所,北京100091 [2]中国农业大学植物保护学院,北京100193 [3]山东农业大学植物保护学院,山东泰安271018

出　　处：《林业科学研究》2024年第2期96-103,共8页Forest Research

基　　金：国家自然科学基金(32230071)。

摘　　要：[目的]通过分析落叶松八齿小蠹伴生长喙壳真菌对寄主的致病能力,探究其对落叶松的危害程度与潜在风险。[方法]利用人工接种的方法,将从落叶松八齿小蠹虫体及坑道中分离到的12种长喙壳真菌接种到长白落叶松上,60 d后观察和测量病斑大小。并对2种Endoconidiophora真菌(E.fujiensis和E.laricicola)诱导寄主抗性的能力进行测定。[结果]结果表明,5种长喙壳真菌E.laricicola、E.fujiensis、Ophiostoma hongxingense、O.peniculi和O.xinganense接种长白落叶松产生的病斑面积大于对照处理,其余7种Ophiostoma真菌产生的病斑面积与对照无显著差异。E.laricicola产生的平均病斑面积最大,为3.51 cm^(2),且诱导寄主萜类物质含量呈现先升高后降低的趋势,表现出对长白落叶松的较强致病力。而E.fujiensis产生的平均病斑面积为1.70 cm^(2),诱导寄主萜类物质含量呈现下降趋势,表现出较弱致病力。[结论]E.laricicola对长白落叶松具有强致病性,E.fujiensis、O.hongxingense、O.peniculi和O.xinganense对长白落叶松具有弱致病性,而另外7种Ophiostoma真菌对落叶松无明显致病性。[Objective]The paper aims to investigate the harm degree and the potential risk of the ophiostomatoid fungi associated with Ips subelongatus by analyzing their pathogenicity.[Method]Through the artificial inoculation,12 species of ophiostomatoid fungi isolated from the body and tunnel of I.subelongatus were inoculated on Larix olgensis.After 60 days,the lesion sizes were observed and measured,and the host resistance responses were tested by inoculation of Endoconidiophora fujiensis and Endoconidiophora laricicola on larch.[Result]The results showed that the area of lesions caused by E.laricicola,E.fujiensis,Ophiostoma hongxingense,Ophiostoma peniculi and Ophiostoma xinganense were larger than those of the control.The lesion areas produced by the other seven species of Ophiostoma showed no significant difference comparing with the control.The average lesion area produced by E.laricicola were the largest with 3.51±0.18 cm^(2),and the content of terpenoids in the host showed a trend of first increasing and then decreasing,showing a strong pathogenicity.However,the average lesion area of E.fujiensis was 1.70±0.08 cm^(2),and the content of host terpenoids showed a decreasing trend,exhibiting weak pathogenicity.[Conclusion]E.laricicola is highly pathogenic to larch,E.fujiensis,O.hongxingense,O.peniculi and O.xinganense are weakly pathogenic,while the other seven Ophiostoma fungi show no obvious pathogenicity.

关 键 词：落叶松八齿小蠹 Endoconidiophora OPHIOSTOMA 单萜 

分 类 号：S763.38[农业科学—森林保护学] S763.15[农业科学—林学]