红瑞木果多糖抗氧化活性的初步研究  

Preliminary Study on the Antioxidant Activity of Polysaccharides from Swida alba Opiz Fruit

作　　者：高长久[1] 李文超[1] 张朝立[1] 孟令锴[1] 王春辉 GAO Changjiu;LI Wenchao;ZHANG Chaoli;MENG Lingkai;WANG Chunhui(College of Pharmacy,Mudanjiang Medical University,Heilongjiang Province,Mudanjiang 157011,China)

机构地区：[1]牡丹江医学院药学院,黑龙江牡丹江157011

出　　处：《中国中医药现代远程教育》2024年第10期139-141,共3页Chinese Medicine Modern Distance Education of China

基　　金：黑龙江省省属高等学校基本科研业务费科研项目【No.2020-KYYWFMY-0050】。

摘　　要：目的通过对红瑞木果多糖抗氧化活性的初步探索,为其后续研究及开发应用提供依据。方法以抗坏血酸(Vc)为阳性对照,分别采用羟基自由基(·OH)清除实验、二苯代苦味肼基自由基(DPPH·)清除实验和三价铁离子(Fe^(3+))还原实验,测定并计算红瑞木果多糖在0.25~1.50 mg/mL浓度范围内对DPPH·、·OH的清除率和对Fe^(3+)的还原力。结果红瑞木果多糖对DPPH·、·OH的清除率和对Fe^(3+)的还原力均有影响,且随浓度增加,其影响作用随之增强。当红瑞木果多糖浓度达到1.50 mg/mL时,其对DPPH·、·OH的清除率和对Fe^(3+)的还原力分别达到79.3%、60.6%和0.565。结论红瑞木果多糖对DPPH·、·OH均有清除作用,对Fe^(3+)有还原作用,是天然有效的抗氧化剂。Objective Through preliminary exploration of the antioxidant activity of the polysaccharides from the Swida alba Opiz fruit,a basis is provided for its subsequent research,development,and application.Methods Using vitamin C(Vc)as a positive control,the hydroxyl radical(·OH)clearance experiment,diphenyl bitter hydrazine radical(DPPH·)clearance experiment,and trivalent iron ion(Fe^(3+))reduction experiment were used to measure and calculate the clearance rate and reducing power of Swida alba Opiz fruit polysaccharide on DPPH·,·OH and Fe^(3+)in the concentration range of 0.25~1.50 mg/mL.Results The polysaccharides from Swida alba Opiz fruit have an impact on the clearance rate of DPPH·,·OH and the reducing power of Fe^(3+),and their effect increases with increasing concentration.When the mass concentration of Swida alba Opiz fruit polysaccharide reaches 1.50 mg/mL,its clearance rate for DPPH·,·OH,and reduction ability for Fe^(3+)reach 79.3%,60.6%,and 0.565.Conclusion Polysaccharide from Swida alba Opiz fruit has a scavenging effect on DPPH·and·OH,and a reducing effect on Fe^(3+).It is a natural and effective antioxidant.

关 键 词：红瑞木果 多糖 抗氧化 实验研究 

分 类 号：R285[医药卫生—中药学]