HPLC同时测定白疕三黄软膏中大黄素和大黄酚的含量  

Simultaneous determination of emodin and chrysophanol in Baibi Sanhuang Ointment by HPLC

作　　者：宋玮 雷晴[1] 王楷 周水涵 余倩颖[1] 米雄飞[1] 陈明岭[1] 肖敏[1] SONG Wei;LEI Qing;WANG Kai;ZHOU Shuihan;YU Qianying;MI Xiongfei;CHEN Minging;XIAO Min(Hospital of Chengdu University of TCM,Chengdu 610072,China)

机构地区：[1]成都中医药大学附属医院,成都610072

出　　处：《中华中医药杂志》2024年第3期1539-1544,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家中医药管理局第二届全国名中医工作室建设项目(No.国中医药人教发[2022]5号);中医药发展补助资金-省名中医工作室(No.500004003);成都中医药大学“杏林学者”学科人才科研提升计划(No.QJRC2021007)。

摘　　要：目的:建立高效液相色谱(HPLC)同时测定白疕三黄软膏中大黄素和大黄酚含量的方法。方法:采用中谱红RD-C_(18)色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸溶液(80∶20)为流动相进行等度洗脱,检测波长为254 nm,柱温为30℃,流速为1.0 mL/min。结果:大黄素、大黄酚分离度良好,其线性范围分别为0.19~6.04μg/mL(r=0.9999)、1.00~31.88μg/mL(r=1.0000);其精密度、重复性及稳定性的RSD≤1.86%;平均回收率为96.8~103.6(RSD≤3.7%);3批白疕三黄软膏中大黄素和大黄酚的平均含量分别为16.78~22.51、117.19~131.65μg/mL。结论:本研究所建方法准确,重复性良好,可快速测定白疕三黄软膏中大黄素和大黄酚的含量,为其质量控制提供参考。Objective:To develop a high performance liquid chromatography(HPLC)method for simultaneous determination of emodin and chrysophanol in Baibi Sanhuang Ointment.Methods:Isocratic elution was performed on a RD-C_(18) column(4.6 mmx250 mm,5μm)with methanol-0.1%phosphoric acid solution(80:20)as mobile phase,the detection wavelength was set at 254 nm,the column temperature was 30 C,and the flow rate was 1.0 mL/min.Results:The separation degree of emodin and chrysophanol were good,their linear range were 0.19-6.04μg/mL(r=0.9999),1.00-31.88μg/mL(r=1.0000),respectively.The RSDs of precision,repeatability,and stability were≤1.86%;and the average recovery were in the range of 96.8-103.6(RSD≤3.7%);The average content ranges of emodin and chrysophanol in 3 batches of Baibi Sanhuang Ointment were 16.78-22.51 and 117.19-131.65μg/mL,respectively.Conclusion:The method developed in this study was accurate,and the repeatability was good,which can quickly achieve determination of emodin and chrysophanol in Baibi Sanhuang Ointment,and to provide reference for the quality control of Baibi Sanhuang Ointment.

关 键 词：白疕三黄软膏 高效液相色谱法 含量测定 大黄素 大黄酚 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]