乳汁菌群对6月龄婴儿肠道菌群的影响初探  

Effects of breast milk microbiota on intestinal microbiota of 6-month-old infants

作　　者：马光宇 罗慧娟[1] 汤小湄[1] 肖小敏[1] MA Guangyu;LUO Huijuan;TANG Xiaomei;XIAO Xiaomin(Department of Gynaecology and Obstetrics,the First Affiliated Hospital of Jinan University,Guangzhou 510632,Guangdong,China)

机构地区：[1]暨南大学附属第一医院妇产科,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2024年第1期21-30,共10页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(81771664)。

摘　　要：目的:基于16S rRNA测序技术初步探讨有菌乳汁对产后6月龄婴儿肠道菌群的影响。方法:本研究共纳入12例产妇及其婴儿,收集产妇产后6月乳汁及其6月龄婴儿粪便标本,根据产后6月乳汁有无菌群,将乳汁分为乳汁无菌组和乳汁有菌组,比较无菌乳汁和有菌乳汁喂养6月龄婴儿肠道菌群多样性与物种差异。结果:收集的12例产后6月乳汁样本中,7例乳汁样本中检测出菌群,乳汁样本中菌群检出率58.33%。有菌乳汁喂养的6月龄婴儿肠道菌群与无菌乳汁喂养6月龄婴儿肠道菌群α多样性和β多样性无显著性差异。有菌乳汁喂养的6月龄婴儿肠道中无害芽孢梭菌、未分类丹毒丝菌科、丹毒丝菌纲、丹毒丝菌目、丹毒丝菌科的相对丰度显著高于无菌乳汁喂养6月龄婴儿(P<0.05),无菌乳汁喂养婴儿肠道中乳酸菌科、乳酸菌属的相对丰度显著高于有菌乳汁喂养的婴儿(P<0.05)。结论:产后6月乳汁中可无菌群的存在,乳汁菌群的有无虽不能改变6月龄婴儿肠道菌群的多样性,但可改变6月龄婴儿肠道菌群的菌群结构。Objective:To investigate the effect of breast milk with bacteria on intestinal microbiota of 6-month-old infants based on 16S rRNA sequencing technology.Methods:A total of 12 postpartum women and their infants were included in this study.The 6-month-old postpartum milk and 6-month-old infant fecal samples were collected.According to the 6-month-old postpartum breast milk with or without bacteria,the breast milk was divided into sterile breast milk group and bacterial breast milk group,and the diversity and species difference of intestinal microbiota of 6-month-old infants fed by sterile breast milk and bacterial breast milk were compared.Results:In 6-month-old postpartum breast milk samples of 12 cases,microbiota was found in 7 cases,and the detection rate of microbiota in breast milk samples was 58.33%.There was no significant difference inαandβdiversity between 6-month-old infants fed with bacterial breast milk and 6-month-old infants fed by sterile breast milk.The relative abundance of Clostridium_innocuum,unidentified_Erysipelotrichaceae,Erysipelotrichia,Erysipelotrichales and Erysipelotrichaceae in intestinal microbiota of 6-month-old infants fed by bacterial breast milk was significantly higher than that of 6-month-old infants fed by sterile breast milk(P<0.05).The relative abundance of Lactobacillaceae and Lactobacillus in intestinal microbiota of infants fed by sterile breast milk was significantly higher than that of infants fed with by bacterial breast milk(P<0.05).Conclusion:The 6-month-old postpartum breast milk may without bacteria.The presence or absence of microbiota in breast milk may not change the diversity of intestinal microbiota of 6-month-old infants,but it can change the structure of intestinal microbiota of 6-month-old infants.

关 键 词：乳汁 乳汁菌群 母乳喂养 婴儿 肠道菌群 

分 类 号：R71[医药卫生—妇产科学]