高能电子束辐照对黄精品质的影响  

Effect of High‐Power Electron Beam Irradiation on Quality of Polygonati rhizoma

作　　者：唐艺文 黄敏[2,3] 付孟 刘靓 王钢 王丹 陈谦[2,3] TANG Yiwen;HUANG Min;FU Meng;LIU Jing;WANG Gang;WANG Dan;CHEN Qian(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,Sichuan,China;Sichuan Institute of Atomic Energy,Chengdu 610101,Sichuan,China;Irradiation Preservation Key Laboratory of Sichuan Province,Chengdu 610101,Sichuan,China)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]四川省原子能研究院,四川成都610101 [3]辐照保藏四川省重点实验室,四川成都610101

出　　处：《食品研究与开发》2024年第7期74-80,共7页Food Research and Development

基　　金：四川省重大科技专项项目(2019ZDZX0003);四川省科技创新人才项目(2021JDRC0042、23CXRC0128);四川省苗子工程项目(2021JDRC0121、2022JDRC0120);四川省区域创新合作项目(2022YFQ0044);成都市龙泉驿区科技计划项目(2022⁃34⁃2)。

摘　　要：以药食同源中药材黄精为原料,采用辐照剂量为0、2、4、6、8 kGy的高能电子束对其进行处理,分析不同辐照剂量下其微生物含量、主要活性物质含量、高效液相色谱(high performance liquid chromatography,HPLC)指纹图谱、色泽、水分、灰分和浸出物等的变化情况,探究高能电子束辐照对黄精品质的作用。结果显示,经高能电子束辐照后,能有效减少黄精中需氧菌、霉菌和酵母菌的总数;低辐照剂量后,黄精活性成分的含量无显著性变化(P>0.05);黄精HPLC指纹图谱相似度均大于0.90,表明经不同辐照剂量的电子束辐照处理后黄精的指纹图谱整体仍较为一致且稳定;当辐照剂量为2、4 kGy时,其色泽与对照组相比无显著性变化(P>0.05);各辐照剂量下黄精的水分、灰分和醇溶性浸出物均符合2020年版《中国药典》的相关规定。综上,当电子束的辐照剂量为2 kGy时,既能使黄精微生物数量明显降低,又能够保障其品质。The Chinese medicinal and edible herb,Polygonati rhizoma,was taken as the raw material and treated with a high⁃power electron beam with irradiation doses of 0,2,4,6,8 kGy.The changes in microbial content,content of main active substances,high⁃performance liquid chromatography(HPLC)fingerprint,color,moisture,ash,and extract were analyzed under different radiation doses,so as to explore the effect of high⁃power electron beam irradiation on the quality of Polygonati rhizoma.The results showed that the total aerobic microbial count,mold count,and saccharomycetes were significantly reduced by the electron beam ir⁃radiation.At low irradiation doses,there was no significant change in the content of active ingredients of Po⁃lygonati rhizoma(P>0.05),and the similarity of HPLC fingerprints of Polygonati rhizoma was greater than 0.90,indicating that the fingerprint of Polygonati rhizoma was consistent and stable after electron beam irra⁃diation with different irradiation doses.When the irradiation dose was 2 kGy and 4 kGy,there was no signifi⁃cant change in color compared with the control group(P>0.05).The content of moisture,ash,and alcohol⁃soluble extracts of Polygonati rhizoma under different irradiation doses was in line with the provisions of the 2020 edition of the Chinese Pharmacopoeia.The electron beam irradiation at a dose of 2 kGy could not only sig⁃nificantly reduce the microbial population in Polygonati rhizoma but also ensure its quality.

关 键 词：电子束辐照 黄精 药食同源 指纹图谱 微生物含量 

分 类 号：R284[医药卫生—中药学]