基孔肯雅病毒(CHIKV)重组痘苗病毒候选疫苗小鼠免疫研究  

Study of immunization in mouse with the CHIKV recombinant vaccinia virus

作　　者：张金鑫 肖妍 李菁菁 韩晶 金宁一 李霄 韩继成[1] ZHANG Jinxin;XIAO Yan;LI Jingjing;HAN Jing;JIN Ningyi;LI Xiao;HAN Jicheng(Academician Workstation,Changchun University of Chinese Medicine,Changchun 133122,China;College of Life Science and Technology,Changchun University of Science and Technology;Changchun Veterinary Research Institute,Chinese Academy of Agricultural Sciences)

机构地区：[1]长春中医药大学科研处院士工作室,吉林长春130122 [2]长春理工大学生命科学技术学院 [3]中国农业科学院长春兽医研究所

出　　处：《中国病原生物学杂志》2024年第4期378-381,共4页Journal of Pathogen Biology

基　　金：吉林省青年科技人才托举工程项目(No.QT202228)。

摘　　要：目的基孔肯雅病毒(Chikungunya virus,CHIKV)是甲病毒属的正股RNA病毒,主要由埃及伊蚊和白纹伊蚊传播,广泛流行于亚洲和非洲的部分地区,每年给全球造成严重的经济损失。本研究基于重组痘苗表达系统构建了CHIKV重组痘苗候选疫苗,进行小鼠免疫评价,为CHIKV疫苗的研发提供理论基础。方法将CHIKV重组痘苗候选疫苗进行小鼠免疫评价,在首次免疫35 d后,收集血清用于特异性抗体检测以及细胞因子检测,分离小鼠脾脏淋巴细胞用于T细胞亚类分型分析,并在35 d时进行攻毒保护实验。结果免疫CHIKV重组痘苗候选疫苗小鼠特异性抗体显著升高,且细胞因子IL-4(P<0.05)和IFN-γ(P<0.05)的表达量显著高于对照组。CD3^(+)CD4^(+)T(P<0.05)和CD3^(+)CD8^(+)T(P<0.05)细胞比例显著高于对照组。攻毒保护实验结果显示,免疫疫苗组体重高于对照组。结论本研究中的CHIKV重组痘苗病毒候选疫苗可以诱导机体产生良好的细胞免疫和体液免疫应答反应,本研究结果可为CHIKV疫苗的研发提供参考。Objective Chikungunya virus(CHIKV)is a positive stranded RNA virus of the Alphavirus,mainly transmitted by Aedes aegypti and Aedes albopictus mosquitoes,which is widely endemic in parts of Asia and Africa,causing serious economic losses worldwide every year.In this study,we constructed a CHIKV recombinant vaccinia virus based on the recombinant vaccinia virus expression system,and performed mouse immunization evaluation to provide a theoretical basis for the development of a CHIKV vaccine.Methods CHIKV recombinant vaccinia virus was evaluated for immunization of mouse.Serum was collected for specific antibody assay as well as cytokine assay,and mouse splenic lymphocytes were isolated for T-cell subclass typing analysis at 35dafter the first immunization,and challenge assay was performed at 35d.The vaccine was used as an antidepressant in the immunization of mouse.Results Mouse immunized with CHIKV recombinant vaccinia virus had significantly higher specific antibodies and significantly higher expression of cytokines IL-4(P<0.05)and IFN-γ(P<0.05)than the control group.The percentages of CD3^(+)CD4^(+)T(P<0.05)and CD3^(+)CD8^(+)T(P<0.05)cells was significantly higher than the control group.The weight of the immunized vaccine group was higher than that of the control group after challenge.Conclusion The CHIKV recombinantvaccinia virus in this study induced good cellular and humoral immune response,and the results of this study may provide a reference for the development of CHIKV vaccine.

关 键 词：基孔肯雅病毒 重组痘苗病毒 细胞免疫 体液免疫 

分 类 号：R392-33[医药卫生—免疫学]