2019-2020年海南省登革病毒E基因序列分析  

Sequence analysis of Dengue virus E gene in Hainan Province,2019-2020

作　　者：王如敏 杜珊珊 陈菁菁[1] 李丹丹[1] 马焱[1] 崔蕾 孙初阳 李建东[2] 任莉娜 WANG Rumin;DU Shanshan;CHEN Jingjing;LI Dandan;MA Yan;CUI Lei;SUN Chuyang;LI Jiandong;REN Lina(Hainan Center for Disease Control and Prevention,Haikou 570203,China;Key Laboratory of Medical Virology,Ministry of Health,National Institute for Viral Disease Control and Prevention,National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,Chinese Center for Disease Control and Prevention)

机构地区：[1]海南省疾病预防控制中心微生物检验室,海南海口570203 [2]中国疾病预防控制中心病毒病预防控制所,传染病溯源预警与智能决策全国重点实验室,卫生部医学病毒和病毒病重点实验室

出　　处：《中国病原生物学杂志》2024年第4期389-394,共6页Journal of Pathogen Biology

摘　　要：目的了解2019-2020年海南省登革病毒(Dengue virus,DENV)E基因序列分子特征,探讨病毒可能的输入来源。方法收集登革热疑似病例血清样本,采用RT-PCR法进行核酸检测并分型,阳性样本进行登革病毒包膜(envelope,E)蛋白基因扩增及测序,应用生物信息软件进行同源性和系统进化分析。结果从49例DENV阳性样本中获得14条DENV E基因序列。进化分析显示,11条DENV1型E基因序列间核苷酸同源性为97.2%~100.0.0%,均属于基因Ⅰ型,其中2条E基因序列(HNLS-8和HNCM-14)与2019年广州流行株(Guangzhou/19XN24503/2019)的核苷酸同源性为100.0%;2条DENV2型E基因序列分别属于Asian 1基因型和Cosmopolitan基因型,其中1条(HNLS-10)与2019-2020年广州流行株(Guangzhou/19XN22111/2019、Guangzhou/19XN50506/2020)同源性为99.6%,另1条(HNLD-2)与2019年昆明2株流行株(Kunming/HNQY20180091/2019、Kunming/HNQY2018005/2019)核苷酸同源性为100.0%。1条DENV3型E基因序列属于基因Ⅲ型,与2018年广州流行株(Guangzhou/18XN61927/2018)和2017年新加坡流行株(Singapore/15095Y15/2017)同源性较高,分别为99.6%和99.5%。结论2019-2020年,海南省检出输入引发的本地传播DENV1、DENV2和DENV3多型病毒,以DENV1为主,显示了加强流动人群中登革热监测与管理,及时防控登革热跨省、跨境传播的重要性。Objective To study the molecular characteristics of Dengue virus(DENV)E gene in Hainan province during 2019-2020,exploring the possible sources of Dengue virus.Methods The serum samples of suspected cases of dengue fever were collected,and the nucleic acid was detected and typed by RT-PCR.The envelope(E)protein gene of dengue virus was amplified and sequenced in the positive samples,use Bioinformatics Software for homology and system evolution analysis.Results 14 DENV E gene sequences were obtained from 49 DENV positive samples.The phylogenetic analysis showed that the nucleotide homology among the 11strains of DENV 1Egene sequences was 97.2%-100%and all belonged to genotype I.Among them,two strains of DENV1Egene sequences(HNLS-8and HNCM-14)have higher nucleotide homology with the Guangzhou Epidemic Strain in 2019(Guangzhou/19XN24503/2019)was 100%.Two strains of DENV2Egene sequences belonged to Asian 1and Cosmopolitan genotype,among them,one E Gene(HNLS-10)has higher nucleotide homology with the Guangzhou epidemic strains in 2019and 2020was 99.6%,another strain(HNLD-2)have higher nucleotide homology with two strains isolated from Kunming in 2019(Kunming/HNQY20180091/2019and Kunming/HNQY2018005/2019)was 100%.One strain of DENV3 E gene belongs to Genotype III,and has higher nucleotide homology with the strains of Guangzhou(Guangzhou/18XN61927/2018),Singapore(Singapore/15095Y15/2017)were 99.6%and 99.5%.Conclusion From 2019to 2020,local transmission of imported DENV1,DENV2,and DENV3 was detected in Hainan Province,with DENV1accounting for the highest proportion,which indicated the necessity of strengthening monitoring and management of dengue fever among mobile populations,for timely prevention and control of cross provincial and cross-border transmission.

关 键 词：登革病毒 包膜蛋白基因 基因型 同源性 

分 类 号：R373[医药卫生—病原生物学]