调控因子Fis对肺炎克雷伯菌致病力影响与机制研究  

The role of fis in the virulence of Klebsiella pneumoniae and the pathogenesis

作　　者：马仁惠 谢继臣 全秋航 张志强 李蓓 MA Renhui;XIE Jichen;QUAN Qiuhang;ZHANG Zhiqiang;LI Bei(School of Basic Medicine,Hubei University of Medicine,Shiyan,Hubei 42000,China;Biomedical Research Institute,Hubei University of Medicine)

机构地区：[1]湖北医药学院基础医学院,湖北十堰442000 [2]湖北医药学院生物医药研究院

出　　处：《中国病原生物学杂志》2024年第4期436-440,共5页Journal of Pathogen Biology

基　　金：湖北省卫生健康委面上项目(No.WJ2021M056);湖北医药学院基础医学院研究生创新基金项目。

摘　　要：目的探究调控因子Fis对肺炎克雷伯菌毒力及致病相关因子的影响。方法构建肺炎克雷伯菌fis基因缺失突变株及回补株,通过体外实验分析肺炎克雷伯菌野生株(WT),fis基因敲除株(Δfis)及回补株(C-fis)的生长能力及生物被膜、荚膜、铁载体合成能力;通过体内小鼠感染实验分析Fis对肺炎克雷伯菌致病力的影响。结果肺炎克雷伯菌fis基因缺失后细菌生长速度明显降低,荚膜合成能力增强,铁载体产生降低,而生物被膜合成能力无差异。通过小鼠腹腔感染实验发现肺炎克雷伯菌fis基因缺失后细菌的致病力下降。结论本研究证实肺炎克雷伯菌Fis调控子可通过影响细菌生长速度、荚膜形成及离子利用而影响细菌的致病力。Objective To explore the effects of regulatory factor Fis on the virulence and pathogenic factors of Klebsiella pneumoniae.Methods The fis gene deletion and replacement strains of K.pneumoniae were constructed.The growth ability of wild WT,fis knockout strain(Δfis)and replacement strain C-fis and their biofilm,capsule and ferric carrier synthesis ability were analyzed by in vitro experiments.Meanwhile,the effect of Fis on the pathogenicity of K.pneumoniae was analyzed by in vivo mouse infection experiment.Results After the deletion of fis gene,the growth rate of K.pneumoniae was significantly decreased,the capsule synthesis ability was enhanced,and the production of iron carrier was decreased,but the biofilm synthesis ability was not different.The pathogenicity of K.pneumoniae decreased after the deletion of fis gene in mice.Conclusion This study confirmed that the Fis regulator of K.pneumoniae can affect the virulence of bacteria by affecting the growth rate,capsule formation and ion utilization of bacteria.

关 键 词：肺炎克雷伯菌 调控因子 FIS 致病性 毒力 

分 类 号：R378[医药卫生—病原生物学]