基于UPLC-Q-Exactive Orbitrap-MS的栀子厚朴汤毒性的代谢组学研究  

Metabolomics Study on Toxicity Mechanisms of Zhi-Zi-Hou-Po Decoction Based on UPLC-Q-Exactive Orbitrap-MS

作　　者：秦依 冯芳[1,2] QIN Yi;FENG Fang(Department of Pharmaceutical Analysis,China Pharmaceutical University,Jiangsu Nanjing 210009;Key Laboratory of Drug Quality Control and Pharmacovigilance(Ministry of Education),Jiangsu Nanjing 210009,China)

机构地区：[1]中国药科大学药物分析教研室,江苏南京210009 [2]药物质量与安全预警教育部重点实验室,江苏南京210009

出　　处：《广州化工》2023年第22期42-45,共4页GuangZhou Chemical Industry

基　　金：国家自然科学基金资助项目(No:81872993)。

摘　　要：基于超高液相色谱-四极杆-静电场轨道阱技术(UPLC-Q-Exactive Orbitrap-MS)结合代谢组学方法研究栀子厚朴汤的毒性机制。评价栀子厚朴汤对大鼠的肝毒性。对采集的两组大鼠肝组织样本进行检测,用主成分分析(PCA)结合正交偏最小二乘判别分析(OPLS-DA)寻找差异代谢物及代谢通路。结果鉴别出30种差异代谢物,涉及9条主要的代谢通路。本研究为栀子厚朴汤的毒性机制研究提供参考。The toxicity mechanism of Zhi-Zi-Hou-Po decoction was studied based on UPLC-Q-Exactive Orbitrap-MS in combination with metabolomic methods.The hepatotoxicity of Zhi-Zi-Hou-Po decoction in rats was evaluated.Samples of rat liver tissue collected were detected,and differential metabolites and metabolic pathways were found by PCA combined with OPLS-DA.As a result,33 different metabolites involving 9 major metabolic pathways were identified.A reference was provided for the study of the toxicity mechanism of Zhi-Zi-Hou-Po decoction.

关 键 词：栀子厚朴汤 毒性 代谢组学 UPLC-Q-Exactive Orbitrap-MS 

分 类 号：R285[医药卫生—中药学]