牛精液低温(0℃)保存的效果研究  

Study on the Effect of Low-temperature(0°C)Preservation of Bovine Semen

作　　者：史瑞莉 何晓霞 牛鹏 龙瑞艳 高庆华[1,3] 方翟[1,3] Shi Ruili;He Xiaoxia;Niu Peng;Long Ruiyan;Gao Qinghua;Fang Di(School of Animal Science and Technology,Tarim University,Alar,843300,Xinjiang;Xinjiang Dingxin Seed Industry Technology Co.,LTD,Aksu,842011,Xinjiang;Key Laboratory of Tarim Animal Husbandry Science and Technology Corps,Alar,843300,Xinjiang)

机构地区：[1]塔里木大学动物科学与技术学院,新疆阿拉尔843300 [2]新疆鼎新种业科技有限公司,新疆阿克苏842011 [3]塔里木畜牧科技兵团重点实验室,新疆阿拉尔843300

出　　处：《中国畜禽种业》2024年第3期11-16,共6页The Chinese Livestock and Poultry Breeding

基　　金：南疆肉牛群体质量提升关键技术研究(2021BB011)。

摘　　要：为研究一种Tris-卵黄稀释液0℃(冰水混合物中)保存牛精液的效果,该试验选用5头西门塔尔牛,将每头牛采集的精液等量分装,按1∶3的比例分别与试验组稀释液(Tris-卵黄专利稀释液)、对照组稀释液(基础稀释液)等温稀释处理,在0℃(冰水混合物中)状态下保存。检测保存0、1、2、4、6、8、10、12 d的精子活率、顶体完整率及质膜完整性,并用保存5 d的精液进行人工授精,统计受胎率。结果表明:保存4~10 d,A组稀释液保存的精子活率显著高于B组(P<0.05);保存10 d时,A组精子活率大于50%;0℃保存牛精子顶体完整率、质膜完整性随保存时间的延长而逐渐降低,但不同处理间无显著差异(P>0.05)。A组稀释液保存牛精子的受胎率显著高于B组。可见,用Tris-卵黄稀释液0℃保存牛精液,保存效果较好,受胎率较高。In order to study the effect of a Tris-egg yolk diluent 0℃(ice water mixture)preservation of bovine semen,the test selected 5 heads of Simensthal cattle,the semen collected from each cattle was divided into equal volumes,and diluted with the test group diluent(Tris-egg yolk patent diluent)and the control group diluent(basic diluent)at a ratio of 1:3,and then preserved at 0℃(ice water mixture).The sperm viability,acrosome integrity and plasma membrane integrity were detected at 0,1,2,4,6,8,10,and 12 d after preservation,and artificial insemination was performed with the semen preserved for 5 d,and the conception rate was calculated.The results showed that:the sperm viability of group A was significantly greater than that of group B after preservation for 4~10 d(P<0.05);the sperm viability of group A was greater than 50%after preservation for 10 d;the acrosome integrity and plasma membrane integrity of bovine sperm preserved at 0℃gradually decreased with the prolongation of preservation time,but there was no significant difference between different treatments(P>0.05).The conception rate of bovine sperm preserved in group A was significantly higher than that of group B.It is clear that the preservation effect of bovine semen preserved at 0℃with Tris-egg yolk diluent is better,and the conception rate is higher.

关 键 词：低温保存 牛精液 稀释液 

分 类 号：S823.3[农业科学—畜牧学]