高压电烧伤对大鼠心肌氧化应激的影响及N-乙酰半胱氨酸的干预作用  

Effects of high-voltage electrical burns on myocardial oxidative stress in rats and the intervention effect of N-acetylcysteine

作　　者：杨文飞 郝嘉文 鲁梦远 赵学刚 李聪颖 盖晨阳 张晶[1] 张庆富 Wenfei Yang;Jiawen Hao;Mengyuan Lu;Xuegang Zhao;Congying Li;Chenyang Gai;Jing Zhang;Qingfu Zhang(Burn and Wound Repair Center,The Third Hospital of Hebei Medical University,Hebei Burn Treatment Technology Innovation Center,Shijiazhuang 050035,China)

机构地区：[1]河北医科大学第三医院烧伤与创面修复中心,河北省烧伤救治技术创新中心,石家庄050035

出　　处：《中华损伤与修复杂志（电子版）》2024年第2期106-112,共7页Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)

基　　金：河北省创新能力提升计划项目(225676120H)。

摘　　要：目的探讨氧化应激在大鼠高压电烧伤心肌损伤中的作用及N-乙酰半胱氨酸(NAC)的干预效果。方法采用随机数字表法将雄性SD大鼠220只分配至假伤组(60只)、电伤组(60只)、盐水组(50只)及NAC组(50只),观测时相为伤后0 h、8 h、24 h、48 h、72 h、1周,相应观测时相点各10只。采用实验高压电击系统,以大鼠左前肢及右后肢分别为电流入口和出口,制作电压3 kV、电击3 s的大鼠高压电烧伤模型。盐水组大鼠腹腔注射生理盐水1 mL/kg;NAC组大鼠腹腔注射10%NAC 1 mL/kg(100 mg/kg)。每组按时相采集心肌组织,检测心肌匀浆上清液超氧化物歧化酶(SOD)活性、丙二醛(MDA)水平、髓过氧化物酶(MPO)水平。光镜下观察心肌组织形态学改变。结果与假伤组比较,电伤组大鼠心肌组织总体SOD活性、MDA水平、MPO水平均增高(均P<0.001)。电伤组SOD活性在伤后48 h高于假伤组(P=0.037),MDA水平在伤后8 h(P=0.009)、24 h(P=0.006)、48 h(P<0.001)、72 h(P<0.001)均高于假伤组,MPO水平在伤后8 h(P<0.001)、24 h(P<0.001)、48 h(P=0.002)、72 h(P=0.008)均高于假伤组。电后1周内,SOD活性先增高后降低,48 h达峰值;MDA先增高后降低,48 h达峰值;MPO逐渐降低。与电伤组各时相相比,盐水组及NAC组SOD活性、MDA水平、MPO水平差异均无统计学意义(均P>0.05)。与假伤组比较,电伤组0~48 h内心肌细胞之间闰盘消失,横纹模糊,细胞水肿,炎性浸润,肌束分离,间质灶性出血;48 h心肌细胞水肿及炎性浸润最明显;48 h~1周心肌组织水肿、炎症等症状逐渐减轻。与电伤组比较,盐水组和NAC组心肌损伤均无明显改善。结论高压电烧伤通过提高大鼠心肌氧化应激水平导致心肌损伤。NAC对大鼠高压电烧伤后心肌组织SOD活性、MDA水平、MPO水平及伤后48 h的心肌损伤干预效果不明显。Objective To investigate the role of oxidative stress in myocardial injury induced by high-voltage electrical burns in rats and the intervention effect of N-acetylcysteine(NAC).Methods A total of 220 male SD rats were randomly divided into sham injury group(n=60),electrical burn group(n=60),saline group(n=50)and NAC group(n=50).The observation time was 0 h,8 h,24 h,48 h,72 h and 1 week after injury,with 10 rats in each group.The experimental high-voltage electrical shock system was used to establish a rat model of high-voltage electrical burn with a voltage of 3 kV and a shock of 3 s,with the left forelimb and right hind limb of rats as the inlet and outlet of current,respectively.Rats in the saline group were intraperitoneally injected with normal saline 1 mL/kg.Rats in NAC group were intraperitoneally injected with 10%NAC 1 mL/kg(100 mg/kg).Myocardial tissue was collected at regular intervals in each group,and superoxide dismutase(SOD)activity,the content of malondialdehyde(MDA)and myeloperoxidase(MPO)in myocardial homogenate supernatant were detected.The morphological changes of myocardium were observed under light microscope.Results Compared with those in sham injury group,the SOD activity,MDA content and MPO content of myocardial tissue in electrical burn group increased(all P<0.001).The activity of SOD in myocardial tissue of rats in electrical burn group was higher than that in sham injury group at post-injury 48 h(P=0.037).The content of MDA in myocardial tissue of rats in electrical burn group was higher than that in sham injury group at post-injury 8 h(P=0.009),24 h(P=0.006),48 h(P<0.001),and 72 h(P<0.001).The content of MPO in myocardial tissue of rats in electrical burn group was higher than that in sham injury group at post-injury 8 h(P<0.001),24 h(P<0.001),48 h(P=0.002),and 72 h(P=0.008).The SOD activity increased at first and then decreased within 1 week after electroacupuncture,and reached the peak at 48 hours.The content of MDA firstly increased and then decreased,and reached the peak at 48 hours

关 键 词：烧伤 电 心肌 氧化应激 乙酰半胱氨酸 大鼠 

分 类 号：R647[医药卫生—外科学]