DNA-AuNCs催化降解有机污染物综合性实验  

Design of a comprehensive experiment for the degradation of nitrogenous organic pollutants by DNA-AuNCs

作　　者：周亭[1] 张霞[1] 王虹[1] 王一蕾[1] 张志庆[1] ZHOU Ting;ZHANG Xia;WANG Hong;WANG Yilei;ZHANG Zhiqing(College of Chemistry and Chemical Engineering,China University of Petroleum(East China),Qingdao 266580,China)

机构地区：[1]中国石油大学(华东)化学化工学院,山东青岛266580

出　　处：《实验技术与管理》2024年第2期114-121,共8页Experimental Technology and Management

基　　金：国家自然科学基金项目(21703286);中央高校基本科研业务费专项资金(22CX03024A);中国石油大学(华东)教学改革项目(CM2022030)。

摘　　要：实验以DNA功能化金纳米簇为催化剂,以硼氢化钠还原对硝基苯酚(4-NP)和甲基橙为模型反应,旨在研究DNA功能化金纳米簇催化降解有机污染物的能力。研究了DNA-AuNCs在不同温度下的催化活性,并对催化剂的动力学和热力学性质进行了系统分析。实验选题紧跟学科前沿发展,涉及纳米科技、有机污染物降解等社会热点问题,实验内容融合了纳米材料、光谱学和催化等理论知识,以及多种仪器的使用,拓展了学生的学术视野,培养了学生的科研素养和创新能力。[Objective]This paper outlines a comprehensive experiment designed to investigate the degradation of nitrogenous organic pollutants by DNA-AuNCs.Organic pollutants,especially nitro-containing organic ones such as nitrophenols and azo dyes are difficult to degrade towing to their stable structures.These pollutants can be absorbed by humans,posing significant risks to both human health and the environment.Therefore,finding efficient ways to remove nitrogen-containing organic pollutants from the environment has become a key research focus.Nitrophenol compounds,with their low electron cloud density in the benzene ring,are challenging to oxidize directly.A more eco-friendly approach is to transform nitrophenol into aminophenol via reduction.Sodium borohydride(NaBH_(4))is a simple and safe reducing agent.However,the mixture of nitrophenol and NaBH_(4) struggles to react quickly,highlighting the need for an effective catalyst to improve the reaction rate.Gold nanoclusters(AuNCs),rapidly developing nanomaterials,have attracted significant attention owing to their excellent surface effect,quantum size effect,unique fluorescence characteristics,and high catalytic activity.AuNCs prepared using DNA as a template have been widely used in the catalytic and biosensing fields thanks to the excellent DNA biocompatibility and its high degree of programmability in terms of length,charge,and sequence.[Methods]With DNA-functionalized AuNCs as catalysts,we investigated their catalytic degradation ability for nitrogenous organic pollutants.This was based on a standard model catalytic reaction of 4-nitrophenol(4-NP)and methyl orange dye pollutants.For instance,4-nitrophenol is a nonbiodegradable noxious organic pollutant.Its reduction product,4-aminophenol,serves as an important intermediate in numerous fields.The catalytic reduction of 4-NP to 4-AP followed the Langmuir-Hinshelwood mechanism.When 4-NP was mixed with NaBH_(4),the color of the mixture turned yellow,and the characteristic absorption peak of 4-NP at 400 nm decreased with t

关 键 词：DNA-金纳米簇 有机污染物 催化性能 综合实验 

分 类 号：O647[理学—物理化学]