Pyronaridine combined with diminazene aceturate inhibits Babesia in vitro and in vivo  

作　　者：Shimaa Abd El-Salam El-Sayed Mohamed Z.Sayed-Ahmed Shaimaa Ahmed Awad Ali Nourah Alsadaan Nawazish Alam Mahmoud S.Alkhoudary Ikuo Igarashi Mohamed Abdo Rizk 

机构地区：[1]National Research Center for Protozoan Diseases,Obihiro University of Agriculture and Veterinary Medicine,Inada-Cho,Obihiro,Hokkaido 080-8555,Japan [2]Department of Biochemistry and Chemistry of Nutrition,Faculty of Veterinary Medicine,Mansoura University,Mansoura 35516,Egypt [3]Department of Clinical Pharmacy,College of Pharmacy,Jazan University,Jazan 45142,Saudi Arabia [4]Medical-Surgical Department,College of Nursing,Jouf University,Sakaka 72388,Saudi Arabia [5]Critical Care and Emergency Nursing,Faculty of Nursing,Mansoura University,Egypt [6]College of Nursing,Jouf University,Sakaka 72388,Saudi Arabia [7]Faculty of Pharmacy,Mansoura University,Mansoura 35516,Dakahlia,Egypt [8]Department of Internal Medicine and Infectious Diseases,Faculty of Veterinary Medicine,Mansoura University,Mansoura 35516,Egypt

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2024年第3期93-99,共7页亚太热带生物医学杂志（英文版）

基　　金：supported by Deputyship for Research&Innovation,Ministry of Education in Saudi Arabia through the project number:ISP23-73.

摘　　要：Objective:To evaluate the combination therapy of pyronaridine tetraphosphate and diminazene aceturate against Babesia in vitro and in vivo.Methods:Bioinformatic analysis was performed using atom pair fingerprints.An in vitro combination test was performed against Babesia bovis and Theileria equi.Moreover,the in vivo chemotherapeutic efficacy of pyronaridine tetraphosphate in combination with diminazene aceturate was investigated against the growth of Babesia microti in mice using a fluorescence inhibitory assay.Results:Pyronaridine tetraphosphate and diminazene aceturate exhibited nearly similar molecular weights.The in vitro combination of pyronaridine tetraphosphate and diminazene aceturate was synergistic on Babesia bovis and additive on Theileria equi.In addition,5 mg/kg pyronaridine tetraphosphate combined with 10 mg/kg diminazene aceturate inhibited Babesia microti growth significantly compared with those observed after treatment with 25 mg/kg diminazene aceturate alone from day 6 post treatment to day 12 post treatment.The combination therapy also normalized the hematological parameters of infected mice.Conclusions:An oral dose of pyronaridine tetraphosphate combined with a subcutaneous dose of diminazene aceturate inhibits Babesia in vitro and in mice,suggesting it might be a new paradigm for the treatment of babesiosis.

关 键 词：BABESIA Pyronaridine tetraphosphate Diminazene aceturate BABESIOSIS THEILERIA 

分 类 号：R285[医药卫生—中药学]