印度梨形孢对香蕉抗旱性的影响  

Effects of Serendipita indica on drought resistance of banana seedlings

作　　者：陈春玲[1] 李金曼 罗杰 陈义挺[2] 程春振 赖钟雄[1] 黄玉吉[1] CHEN Chunling;LI Jinman;LUO Jie;CHEN Yiting;CHENG Chunzhen;LAI Zhongxiong;HUANG Yuji(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fruit Research Institute of Fujian Academy of Agricultural Sciences,Fuzhou 350002,China;College of Horticulture,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002 [2]福建省农业科学院果树研究所,福州350002 [3]山西农业大学园艺学院,太古030801

出　　处：《应用与环境生物学报》2024年第1期118-125,共8页Chinese Journal of Applied and Environmental Biology

基　　金：福建农林大学科技创新专项基金项目(CXZX2020026A,KFb22027XA);国家重点研发计划项目(2019YFD1000900);国家现代农业产业技术体系建设(香蕉)专项资金项目(GARS-31-15);福建省高原学科建设经费项目(102/71201801101)资助。

摘　　要：为了解印度梨形孢(Serendipita indica)对香蕉干旱胁迫下生长的影响以及提高香蕉抗旱性的生理生化机制,对正常浇水和干旱胁迫下的幼苗进行不加S.indica和加S.indica处理,比较香蕉苗表型、各项生长指标及相关生理生化指标的差异.结果显示,当土壤含水量适宜香蕉幼苗生长时,接种S.indica的香蕉苗仅叶片数和茎粗显著高于CK组,其他各项生长指标与CK组无显著差异;干旱胁迫时,接种S.indica的各项生长指标均优于未接种S.indica组,其中接种S.indica的根数量显著多于未接种S.indica,约为2.19倍(P<0.01),接种S.indica的干旱症状比未接种的香蕉苗轻,出现干旱症状的时间滞缓,接种S.indica的根中过氧化氢酶(CAT)活性为未接种S.indica组的1.58倍,叶片中过氧化物酶(POD)活性、丙二醛(MDA)含量、脯氨酸(Pro)含量、可溶性糖(SS)含量、脱落酸(ABA)、叶绿素含量分别为未接种S.indica的1.78倍、50.23%、1.61倍、1.83倍、1.39倍和1.15倍,均达到极显著水平(P<0.01).本研究表明干旱胁迫时,印度梨形孢主要通过提高香蕉叶片中POD活性和根系中CAT活性来降解、清除干旱胁迫产生的有害物质,减少香蕉叶片和根系中MDA的积累,促进香蕉叶片中Pro、SS合成及积累,减缓叶绿素的分解,加速ABA的合成与积累,进而提高香蕉抗旱性.This study aimed to study the effects of Serendipita indica colonization on the growth characteristics of banana seedlings under drought stress and to reveal the physiological and biochemical mechanisms underlying these effects.The normally watered and under drought stress‘Tianbaojiao’seedlings were treated with-and without-S.indica colonization,respectively.We compared the difference in phenotypes,growth indexes,the activity of superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD),the malondialdehyde content(MDA),proline(Pro),soluble sugar(SS)in leaves and roots and the content of chlorophyll,abscisic acid(ABA)in leaves.The results showed that when the soil water content was suitable for the growth of banana seedlings,only the number of leaves and stem diameters of banana seedlings with S.indica colonization were significantly higher than those of the control group.There were no significant differences in other growth indexes between the two groups.Under drought stress treatment,the phenotypic variation of seedlings with S.indica colonization was less severe than those without S.indica colonization,and drought symptoms appeared later.The growth indexes of seedlings with S.indica colonization were all better than those without S.indica colonization.The number of roots with S.indica colonization was significantly more than those without S.indica colonization,about 2.19 times(P<0.01).The catalase(CAT)activity in roots with S.indica colonization was 1.58 times that of those without colonization.The activity of peroxidase(POD),the malondialdehyde content(MDA),proline(Pro),soluble sugar(SS),abscisic acid(ABA),and chlorophyll in leaves of seedlings with S.indica colonization were 1.78 times,50.23%,1.61 times and 1.83 times that of those without colonization,respectively.These findings were all significant(P<0.01).It was concluded that Serendipita indica could improve the drought resistance of bananas by improving the activity of POD in leaves and the activity of CAT in roots to degrade and remove harmful subs

关 键 词：干旱胁迫 印度梨形孢 生长 生理生化 抗旱 

分 类 号：S668.1[农业科学—果树学]