贵州南瓜蒂药材质量标准提升研究  

Study on Improving the Quality Standard of Guizhou Cucurbita moschata

作　　者：傅建 赵燕云 刘艳飞 李美位 张前飞 FU Jian;ZHAO Yanyun;LIU Yanfei;LI Meiwei;ZHANG Qianfei(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Guiyang Food and Drug Inspection and Testing Center,Guiyang 550081,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]贵阳市食品药品检验检测中心,贵州贵阳550081

出　　处：《中国民族民间医药》2024年第6期53-56,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：贵州省食品药品监督管理局中药材;民族药材质量标准修订项目(DBYC52014)。

摘　　要：目的:为进一步提升贵州南瓜蒂药材及饮片的质量标准。方法:根据《中国药典》2020版第四部通用的测定方法进行试验,运用通则0832水分测定的方法测定南瓜蒂饮片,运用通则2303灰分测定法对南瓜蒂饮片的总灰分与酸不溶性灰分进行测定,运用通则0502薄层色谱法定性分析南瓜蒂中豆甾醇,运用通则0512高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定南瓜蒂中豆甾醇的含量。结果:贵州几个不同地区南瓜蒂水分含量为7.401%~11.541%,测得其总灰分含量为3.142%~4.277%,酸不溶性灰分含量为0.7298%~1.141%,南瓜蒂中豆甾醇的含量为0.0361~0.1677μg/g。结论:通过测定贵州不同地区南瓜蒂水分、总灰分、酸不溶性灰分、豆甾醇的含量,为南瓜蒂的药材质量控制提供参考依据。Objective To further improve the quality standard of Guizhou Cucurbita moschata.Methods The moisture,total ash content and acid insoluble ash content of Guizhou Cucurbita moschata were determined according to the 2020 edition of Chinese pharmacopoeia.The effective components in the samples was identified by TLC.The stigmasterol in the samples was determined by high performance liquid chromatography(HPLC-ELSD).Results The moisture content of Guizhou Cucurbita moschata ranged from 7.401%to 11.541%,the total ash content ranged from 3.142%to 4.277%,and the acid insoluble ash content ranged from 0.716%to 1.141%.The stigmasterol was detected by TLC.The HPLC-ELSD was employed to determine the content of stigmasterol in Cucurbita moschata for the first time.The results showed that the content ranged from 0.0361-0.1677 g/g.Conclusion The moisture,total ash,acid insoluble ash and the content of stigmasterol can provide reference for quality control of Cucurbita moschata.

关 键 词：南瓜蒂 质量标准 化学成分 含量测定 

分 类 号：R284.2[医药卫生—中药学]