当归挥发油对小鼠抑郁行为的影响  

Effects of Angelica sinensis Volatile Oil on Depressive Behavior in Mice

作　　者：谢云亮 张博 XIE Yunliang;ZHANG Bo(Department of Neurology,Suzhou High-tech Zone People’s Hospital,Suzhou 215129,China;Office of Medical Association,Suzhou High-tech Zone People’s Hospital,Suzhou 215129,China)

机构地区：[1]苏州高新区人民医院神经内科,江苏苏州215129 [2]苏州高新区人民医院医联体办公室,江苏苏州215129

出　　处：《现代食品科技》2024年第3期9-17,共9页Modern Food Science and Technology

基　　金：吉林省教育厅科技项目(JJKH20210059KJ,JJKH20210055KJ)。

摘　　要：通过建立慢性轻度不可知应激刺激(CUMS)诱导的小鼠抑郁模型,研究当归挥发油对抑郁行为的影响及机制。小鼠随机分为正常组、模型组、低、中、高剂量当归挥发油组(15、30、60 mg/kg)及盐酸氟西汀组(2.1 mg/kg)。干预4周后发现,与模型组比较,当归挥发油各剂量组小鼠中央穿越次数、中央区活动时间及糖水偏好度升高(P<0.01),悬尾不动时间下降(P<0.05,P<0.01),海马CA1区病理变化减轻,血清白细胞介素-1β(IL-1β)、IL-6及肿瘤坏死因子-α(TNF-α)含量降低(P<0.01),而IL-10含量增加(P<0.01);除低剂量当归挥发油组小鼠脑组织多巴胺(DA)含量及神经营养蛋白-3(NT-3)表达无明显变化外(P>0.05),剩余各组小鼠DA、去甲肾上腺素(NE)、5-羟色胺(5-HT)含量及神经生长因子(NGF)、NT-3、脑源性神经营养因子(BDNF)表达均升高(P<0.05,P<0.01)。上述结果表明,当归挥发油具有改善CUMS诱导的小鼠抑郁行为作用,该作用与抑制神经炎症、上调单胺类神经递质含量及促进神经营养因子表达有关。To determine the effects and mechanism of Angelica sinensis volatile oil(ASVO)on depressive behavior,chronic unpredictable mild stress(CUMS)-induced depression mouse models were established.Mice were divided into normal,model,low-dose,medium-dose,and high-dose ASVO(15,30,and 60 mg/kg,respectively),and fluoxetine hydrochloride(2.1 mg/kg)groups.After four weeks of intervention,the number of central zone crossing,central zone activity duration,and sucrose preference were increased(P<0.01)while the tail suspension time was reduced(P<0.05,P<0.01)in all ASVO groups compared with those in the model group.The pathological changes in the hippocampal CA1 region were alleviated,and the contents of serum interleukin-1β(IL-1β),IL-6,and tumor necrosis factor-α(TNF-α)decreased(P<0.01)while that of IL-10 increased(P<0.01).Despite no significant changes in the dopamine(DA)content and neurotrophin-3(NT-3)expression in the brain tissue of mice in the low-dose ASVO group(P>0.05),the contents of DA,norepinephrine(NE),and 5-hydroxytryptamine(5-HT)and the expression of nerve growth factor(NGF),NT-3,and brain-derived neurotrophic factor(BDNF)increased in the other groups(P<0.05,P<0.01).These results indicate that ASVO can improve CUMS-induced depressive behavior in mice,which is related to the inhibition of neuroinflammation,upregulation of monoamine neurotransmitter contents,and promotion of neurotrophic factor expression.

关 键 词：当归挥发油 抑郁 炎症 单胺类神经递质 神经营养因子 

分 类 号：R285.5[医药卫生—中药学]