天香丹抑制P2X7/NLRP3表达改善动脉粥样硬化的作用机制研究  

The Effect of Tianxiangdan on Atherosclerosis by Inhibiting P2X7/NLRP3 Expression

作　　者：白银雪 张夏夏 吴丹丹 任珊 安冬青[1] BAI Yinxue;ZHANG Xiaxia;WU Dandan;REN Shan;AN Dongqing(Xinjiang Medical University,Key Laboratory of Xinjiang Famous Physicians and Characteristic Prescriptions,Urumchi 830054,Xinjiang,China)

机构地区：[1]新疆医科大学,新疆名医名方与特色方剂学重点实验室,乌鲁木齐830054

出　　处：《中西医结合心脑血管病杂志》2024年第7期1225-1228,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然科学基金项目(No.82060861);新疆维吾尔自治区重点实验室项目(No.2020D04006);新疆维吾尔自治区重大科技专项项目(No.2022A03019)。

摘　　要：目的:探讨天香丹对动脉粥样硬化小鼠嘌呤能配体门控离子通道7(P2X7)、核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)的影响。方法:选取8周龄无特定病原体(SPF)级雄性载脂蛋白E基因敲除(ApoE-/-)小鼠30只,予以高脂饲料喂养12周,造模成功后随机分为模型组、阿托伐他汀组、天香丹组,每组10只;另选取C57BL/6J小鼠10只予普通饲料喂养,设为空白对照组。酶联免疫吸附法(ELISA)检测血清白细胞介素(IL)-18、IL-1β、腺嘌呤核苷三磷酸(ATP)的水平;免疫组化检测组织中P2X7、NLRP3的表达。结果:ELISA结果显示:与空白对照组相比,模型组IL-18、IL-1β、ATP水平升高(P<0.05);与模型组相比,阿托伐他汀组、天香丹组IL-18、IL-1β、ATP水平降低(P<0.05)。免疫组化结果显示:与空白对照组相比,模型组P2X7、NLRP3表达均明显升高(P<0.05);与模型组相比,阿托伐他汀组、天香丹组P2X7、NLRP3表达均明显降低(P<0.05)。结论:天香丹改善动脉粥样硬化与调节钾稳态抑制炎症小体的激活有关。Objective:To investigate the effect of Tianxiangdan on purinergic ligand-gated ion channel 7(P2X7)and nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)in atherosclerotic mice.Methods:Thirty 8-week-old SPF male ApoE-/-mice were selected and fed with high-fat diet for 12 weeks.After successful modeling,they were randomly divided into model group,statin group,and Tianxiangdan group,with 10 mice in each group.Ten C57BL/6J mice were fed with normal diet as blank control group.The levels of interleukin(IL)-18,IL-1β,and adenosine triphosphate(ATP)in serum were detected by enzyme-linked immunosorbent assay(ELISA).The expression of P2X7 and NLRP3 in tissues was detected by immunohistochemistry.Results:ELISA results displayed that compared with the blank group,the levels of IL-18,IL-1β,and ATP in model group increased(P<0.05).Compared with the model group,the levels of IL-18,IL-1β,and ATP in statin group and Tianxiangdan group decreased(P<0.05).The results of immunohistochemistry showed that the expression of P2X7 and NLRP3 in model group was significantly higher than that in blank group(P<0.05).The expression of P2X7 and NLRP3 in the statin group and Tianxiangdan group decreased compared with the model group(P<0.05).Conclusion:The improvement of atherosclerosis by Tianxiangdan is related to regulating potassium homeostasis and inhibiting the activation of inflammasome.

关 键 词：动脉粥样硬化 天香丹 核苷酸结合寡聚化结构域样受体蛋白3 嘌呤能配体门控离子通道7 腺嘌呤核苷三磷酸 实验研究 

分 类 号：R285.5[医药卫生—中药学]