基于网络药理学薤白治疗冠状动脉粥样硬化性心脏病心绞痛的作用机制分析  

Mechanism analysis of treating angina pectoris of coronary heart disease with Xiebai based on network pharmacology

作　　者：金爱林 吴锋 舒华 JIN Ailin

机构地区：[1]贵州中医药大学,贵州贵阳550001 [2]贵州中医药大学第二附属医院,贵州贵阳550001

出　　处：《中医临床研究》2024年第5期46-51,共6页Clinical Journal Of Chinese Medicine

基　　金：国家自然基金(81760867);贵州省中医药管理局项目(QZYY2017-033);贵州省卫生健康委科学技术基金(gzwkj2022-040);贵州中医药大学第二附属医院院内科研项目(GZEYK2020-31号)。

摘　　要：目的:从网络药理学出发,研究薤白对冠状动脉粥样硬化性心脏病(简称冠心病)心绞痛的作用机制,为新药研发和经典方剂在临床上的拓展运用提供借鉴。方法:通过中药系统药理学数据库与分析平台(TCMSP),获得薤白中的主要化合物组成及相应靶点;通过DisGeNET,GeneCards等数据库获得冠心病心绞痛的主要作用靶点,取疾病与药物之间的交集靶点,然后通过STRING平台建立薤白对冠心病心绞痛疗效确切靶点的蛋白质-蛋白质相互作用网络图,发掘该网络潜在蛋白质功能模块,将数据导入DAVID数据库中,用于基因本体论(GO)功能富集分析及京都基因与基因组百科全书(KEGG)通路富集分析。结果:筛选出薤白中11种为有效化学成分,876个有效靶点,冠心病心绞痛疾病靶点4282个,薤白治疗冠心病心绞痛的关键靶点24个。结果表明靶点丝氨酸/苏氨酸蛋白激酶1(Akt Serine/Threonine Kinase 1,AKT1),过氧化物酶体增殖物激活受体γ(Peroxisome Proliferator Activated Receptor Gamma,PPARG),半胱氨酸蛋白酶3(Caspase 3,CASP3)和前列腺素氧化环化酶2(Prostaglandin-Endoperoxide Synthase 2,PTGS2)与其他蛋白有很好的关联,说明以上靶点为薤白治疗冠心病心绞痛的关键靶点。GO分析中得出与疾病最相关的11个条目,主要有氧化应激、凋亡过程的正向转控、细胞质、腺苷三磷酸(Adenylate triphosphate,ATP)酶结合、转录辅激活子结合以及序列特异性DNA结合;KEGG富集通路分析得出与疾病最相关的6条通路,主要有脂质和动脉粥样硬化、过氧化物酶体增殖物激活受体、丝裂原活化蛋白激酶、白细胞介素-17、缺氧诱导因子-1、肿瘤坏死因子等信号通路。结论:该研究表明薤白在治疗冠心病心绞痛中具有多成分、多靶点、多通路等特点,可为薤白以后在临床的利用开发提供参考。Objective:From the perspective of network pharmacology,the mechanism of Xiebai(Bulbus Allii Macrostemonis)in treating coronary heart disease angina pectoris was studied to provide reference for new drug development and the clinical application of classic prescriptions.Methods:The main compound components and corresponding targets in Xiebai were acquired through TCMSP database.The main targets of coronary heart disease angina pectoris were obtained through databases such as DisGeNET and GeneCards.The intersection targets between disease and drug were taken,and then the protein-protein interaction network diagram of Xiebai on the exact targets of angina pectoris was established through the STRING platform,and the potential protein functional modules were excavated.The data was imported into DAVID database for gene ontology(GO)functional enrichment analysis and Kyoto EncyclopediaOofGenes and Genomes(KEGG)pathway enrichment analysis.Results:A total of 11 active chemical components and 876 effective targets were screened out in Xiebai,and 4282 disease targets were obtained in angina pectoris,and 24 key targets of Xiebai in treating coronary heart disease angina pectoris were found.The results showed that the targets AKT1,PPARG,CASP3,and PTGS2 are well associated with other proteins,indicating that these targets are key targets for Xiebai to treat angina pectoris.GO analysis revealed 11 entries most relevant to the disease,which may be associated with oxidation stress,positive regulation of apoptosis,cytoplasm,ATP binding,coactivator binding,and sequence-specific DNA binding.KEGG pathway enrichment analysis revealed 6 pathways most closely related to the disease,mainly including lipid metabolism and atherosclerosis,PPAR,MAPK,IL-17,HIF-1 and TNF signaling pathways.Conclusion:This study shows that Xiebai granules in the treatment of coronary heart disease angina pectoris has characteristics such as multi-components,multi-targets,and multi-pathways,which can lay a foundation for the future clinical utilization and develop

关 键 词：网络药理学 薤白 冠状动脉粥样硬化性心脏病心绞痛 作用机制 

分 类 号：R541.4[医药卫生—心血管疾病]