中药怀牛膝醇提物有效单体成分的定性定量研究  

Qualitative and quantitative studies on the effective monomers of the alcohol extract of Huainiuxi

作　　者：朱厚均 马笃军[2] 刘乐诗 赵静[1] 彭力平[2] 余阗[2] ZHU Houjun

机构地区：[1]广州中医药大学,广东广州510006 [2]广州中医药大学第四临床医学院,广东深圳518033

出　　处：《中医临床研究》2024年第7期55-61,共7页Clinical Journal Of Chinese Medicine

基　　金：国家自然科学基金项目(81804124);广东省中医药管理局课题(20232154);深圳市三名工程院内项目(SMGC20220704)。

摘　　要：目的:基于现代检测技术对怀牛膝有效制剂——牛膝醇提物的单体成分进行定性定量分析。方法:将怀牛膝醇提物液体制剂按照检测进样要求分别制备成烘干物和液体物,再应用核磁共振波谱(Nuclear Magnetic Resonance,NMR)、气相色谱质谱联用(Gas Chromatography-Mass Spectrometry,GC-MS)、顶空-气相色谱质谱联用(Headspace-Gas Chromatography-Mass Spectrometry,HS-GCMS)、液相色谱质谱联用(Liquid Chromatography-Mass Spectrometry,LC-MS)、三重串联四极杆液相质谱联用(Triple Series Quadrupole Liquid Mass Spectrometry,LC-MSMS)等技术对牛膝醇提物的化学成分进行鉴定分析。结果:共检测出23个单体成分,包括糖类1个,类黄酮类1个,黄酮类1个,甾酮类1个,三萜类1个,氨基酸类16个,果酸类2个,其中槲皮素含量为0.0021%,β-蜕皮甾酮含量为0.0210%,齐墩果酸含量为0.00017%,并根据分子片段信息预测出了4’,5,6,7-四甲氧基黄酮成分。结论:本实验为怀牛膝醇提物制剂的临床应用提供了有效单体成分参考,为进一步对其单体物质的“药物-疾病-靶点”进行验证研究和开发应用提供依据。Objective:The monomer components of alcohol extract of Huainiuxi(Radix Achyranthis Bidentatae),an effective preparation of Huainiuxi,were qualitatively and quantitatively analyzed based on modern detection technology.Methods:The liquid preparation of alcohol extract of Huainiuxi was prepared into drying substance and liquid substance respectively according to the requirements of detection and injection.And then the chemical constituents of alcohol extract of Huainiuxi were identified and analyzedO by nuclear magnetic resonance(NMR),gas chromatography-mass spectrometry(GC-MS),headspace-gas chromatography-mass spectrometry(HS-GCMS),liquid chromatography-mass spectrometry(LC-MS)and triple series quadrupole liquid mass spectrometry(LC-MSMS)and so on.Results:A total of 23 components were detected,including 1 carbohydrate,1 flavonoid,1 anthoxanthin,1 sterone,1 triterpene,16 amino acids and 2 fruit acids.The contents of quercetin,β-ecdysterone and oleanolic acid were 0.0021%,0.0210%and 0.00017%,respectively.According to the molecular fragments,the components of 4’,5,6,7-Tetramethoxyflavone were predicted.Conclusion:This experiment provides a reference for the clinical application of effective monomer composition of alcohol extract of Huainiuxi,and provides a basis for further verification,research,development and application of drug-disease-target of its monomer components.

关 键 词：怀牛膝 牛膝醇提物 定性定量 质谱仪 单体成分 

分 类 号：R284[医药卫生—中药学]