iTRAQ-based proteomics reveals the mechanism of action of Yinlai decoction in treating pneumonia in mice consuming a high-calorie diet  

作　　者：Qianqian Li Tiegang Liu Chen Bai Xueyan Ma Hui Liu Zi’an Zheng Yuxiang Wan He Yu Yuling Ma Xiaohong Gu 

机构地区：[1]School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China [2]Institute of Chinese Medicine Epidemic Disease,Beijing University of Chinese Medicine,Beijing 102488,China [3]School of Traditional Chinese Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China [4]Department of Acupuncture and Mini-invasive Oncology,Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China [5]OCMRC Center,University of Oxford,Oxford OX13PT,United Kingdom

出　　处：《Journal of Traditional Chinese Medical Sciences》2024年第1期21-32,共12页中医科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(81874421);the Innovation Team and Talents Cultivation Program of the National Administration of Traditional Chinese Medicine(ZYYCXTD-C-202006).

摘　　要：Objective:To uncover the underlying mechanisms of action of the Yinlai decoction on high-calorie dietinduced pneumonia through proteomics analysis.Methods:Based on the Gene Expression Omnibus(GEO)database,lung tissue samples from normal and high-fat diet(HFD)fed mice in the GSE16377 dataset were selected as test cohorts to identify differentially expressed genes and conduct bioinformatics analyses.In the animal experiments,mice were randomly divided into the control(N),high-calorie diet pneumonia(M),and Yinlai decoction treatment(Y)groups.Mice in the M group received high-calorie feed and a 0.5 mg/mL lipopolysaccharide solution spray for 30 min for 3 d.The mice in the Y group were intragastrically administered 2 mL/10 g Yinlai decoction twice daily for 3 d.Pathological evaluation of the lung tissue was performed.Differentially expressed proteins(DEPs)in the lung tissue were identified using quantitative proteomics and bioinformatics analyses.The drug-target relationships between Yinlai decoction and core DEPs in the lung tissue were verified using AutoDock Vina and Molecular Graphics Laboratory(MGL)Tools.DEPs were verified by western blot.Results:GEO data mining showed that an HFD altered oxidative phosphorylation in mouse lung tissue.The Yinlai decoction alleviated pathological damage to lung tissue and pneumonia in mice that were fed a high-calorie diet.A total of 47 DEPs were identified between the Y and M groups.Enrichment analysis revealed their association with energy metabolism pathways such as the tricarboxylic acid cycle(TCA)and oxidative phosphorylation.The protein-protein interaction network revealed that Atp5a1,Pdha1,and Sdha were the target proteins mediating the therapeutic effects of Yinlai decoction.Molecular docking results suggested that the mechanism of the therapeutic effect of Yinlai decoction involves the binding of brassinolide,praeruptorin B,chrysoeriol,and other components in Yinlai decoction to Atp5a1.Conclusion:The Yinlai decoction alleviated lung tissue damage and pneumonia in mice tha

关 键 词：Yinlai decoction High-calorie diet PNEUMONIA iTRAQ proteomics Energy metabolism 

分 类 号：R285.5[医药卫生—中药学]