枸杞多糖通过调节线粒体功能缓解H_(2)O_(2)所致的SH-SY5Y细胞损伤  

SH-SY5Y Cell Damage Caused by Lycium Barbarum Polysaccharide Alleviating H_(2)O_(2) by Regulating Mitochondrial Function

作　　者：刘怀绢 李星愿 陆心如 梁林梅 李香容 张艺雯 张淑蓉 郭敏芳[1] LIU Huaijuan;LI Xingyuan;LU Xinru;LIANG Linmei;LI Xiangrong;ZHANG Yiwen;ZHANG Shurong;GUO Minfang(Medical School,Shanxi Datong University,Datong Shanxi,037009)

机构地区：[1]山西大同大学医学院,山西大同037009

出　　处：《山西大同大学学报（自然科学版）》2024年第2期96-100,共5页Journal of Shanxi Datong University(Natural Science Edition)

基　　金：山西大同大学2022年大学生创新创业训练计划项目[XDC2022174];山西省基础研究计划[20210302123476]

摘　　要：目的基于线粒体损伤探讨枸杞多糖(LBP)缓解过氧化氢(H_(2)O_(2))所致的SH-SY5Y细胞损伤的机制。方法将人神经母细胞瘤SH-SY5Y细胞分为PBS对照组、H_(2)O_(2)组和H_(2)O_(2)+LBP组。MTT法检测细胞活性,TUNEL染色检测细胞凋亡,ATP检测试剂盒评估ATP水平,免疫荧光染色检测caspase3的表达,Western blot法检测p-Drp1、OPA1、NDUFB8、SDHB、MTCO1、ATP5A和UQCRC2的表达。结果与对照组相比,H_(2)O_(2)组细胞活性下降,凋亡率增加,ATP水平下降,caspase3和p-Drp1表达增加,OPA1、NDUFB8、SDHB、MTCO1、ATP5A和UQCRC2表达均减少;与H_(2)O_(2)组相比,LBP干预后上述指标呈相反趋势。结论LBP能够通过调节线粒体动力学,改善线粒体功能,缓解H_(2)O_(2)所致的神经元损伤。Objective To explore the mechanism of SH-SY5Y cell injury caused by lycium barbarum polysaccharide(LBP)alleviating hydrogen peroxide(H_(2)O_(2))based on mitochondrial damage.Method Human neuroblastoma SH-SY5Y cells were divided into three groups:PBS control group,H_(2)O_(2)group,and H2O2+LBP group.MTT assay was used to detect cell activity;TUNEL staining was used to detect cell apoptosis;ATP detection kit was used to evaluate ATP levels;Immunofluorescence staining was used to detect the expression of caspase3;Western blot was used to detect the expression of p-Drp1,OPA1,NDUFB8,SDHB,MTCO1,ATP5A,and UQCRC2.Result Compared with the control group,the cell activity of H_(2)O_(2)group decreased;the apoptosis rate increased,the ATP level decreased,the expression of caspase3 and p-Drp1 increased,the expression of OPA1,NDUFB8,SDHB,MTCO1,ATP5A and UQCRC2 decreased;Compared with the H_(2)O_(2)group,after LBP intervention,the above indicators showed an opposite trend.Conclusion LBP can regulate mitochondrial dynamics,improve mitochondrial function,and alleviate neuronal damage caused by H_(2)O_(2).

关 键 词：枸杞多糖 线粒体 过氧化氢 神经元 

分 类 号：R285.5[医药卫生—中药学]