基于UPLC-Q-Exactive Plus-Orbitrap MS的丹参提取物成分表征及其抗血栓作用谱效相关药效物质基础解析  

Component characterization of Salvia miltiorrhiza extracts based on UPLC-QExactive Plus-Orbitrap MS and analysis of pharmacodynamic material basis related to spectral effect of its antithrombotic activity

作　　者：刘悦 丁晓彦[3] 王娜 高燕[1] 杨龙飞[1] 吕婧[1] 韩利文 傅春升[6] 赵渤年 LIU Yue;DING Xiaoyan;WANG Na;GAO Yan;YANG Longfei;LYU Jing;HAN Liwen;FU Chunsheng;ZHAO Bonian(Collaborative Innovation Center for Ecological Protection and High Quality Development of Characteristic Traditional Chinese Medicine in Yellow River Basin,Institute of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Department of Pharmacy,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China;Shandong Academy of Chinese Medicine,Jinan 250014,China;Shandong Xiandai University,Jinan 250104,China;School of Pharmaceutical Sciences(Institute of Materia Medica),Shandong First Medical University(Shandong Academy of Medical Sciences),Jinan 250024,China;Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China)

机构地区：[1]山东中医药大学药物研究院,黄河流域特色中药生态保护和高质量发展协同创新中心,山东济南250355 [2]山东中医药大学附属医院药学部,山东济南250014 [3]山东省中医药研究院,山东济南250014 [4]山东现代学院,山东济南250104 [5]山东第一医科大学(山东省医学科学院),药学院(药物研究所),山东济南250024 [6]山东中医药大学附属医院,山东济南250014

出　　处：《中草药》2024年第5期1609-1619,共11页Chinese Traditional and Herbal Drugs

基　　金：山东省重点研发计划子课题(2021CXGC010511-01-005);山东省自然科学基金联合基金项目(ZR2021LZY021)。

摘　　要：目的研究不同产地丹参Salvia miltiorrhiza提取物化学成分与其抗血栓作用的谱效关系,分析丹参提取物的抗血栓活性成分。方法采用超高效液相色谱-四极杆-静电轨道阱-高分辨质谱(UPLC-Q-Exactive Orbitrap MS)法建立丹参提取物的指纹图谱;结合层次聚类分析(hierarchical cluster analysis,HCA)、正交偏最小二乘-判别分析(orthogonal partial least squares-discrimination analysis,OPLS-DA)探讨不同产地丹参的成分差异;以斑马鱼为模式生物测定不同产地丹参提取物的抗血栓活性;利用偏最小二乘回归(partial least squares regression,PLSR)分析进行谱-效相关研究,筛选出药效贡献较大成分。结果通过聚类分析,60批丹参样品聚为2类;OPLS-DA筛选出2组间的15个差异共有峰;PLSR分析提示在标定的39个共有峰中,与丹参抗血栓活性最相关的化合物可能为峰1(二糖)、峰31(新隐丹参酮)、峰26(丹参醛)、峰34(隐丹参酮)、峰39(二氢隐丹参酮)、峰8(迷迭香酸)、峰18(4-甲基丹酚酸酯B或异构体)和峰17(丹酚酸B)。结论借助中药谱效学初步阐释了丹参抗血栓作用的药效物质基础,为丹参谱-效相关质量评价体系的建立提供科学依据,为丹参临床治疗血栓性疾病提供数据支持。Objective To study the spectral effect between chemical components of Salvia miltiorrhiza extracts from different origins and their antithrombotic activity,and analyze the antithrombotic active components of S.miltiorrhiza extracts.Methods The fingerprints of S.miltiorrhiza extracts were established by UPLC-Q-Exactive-Orbitrap MS;The ingredient differences of S.miltiorrhiza from different origins were investigated through hierarchical cluster analysis(HCA)and orthogonal partial least squaresdiscriminant analysis(OPLS-DA);Zebrafish was used as a model to determine the antithrombotic activity of S.miltiorrhiza extracts from different origins;Partial least squares-discriminant(PLSR)analysis was applied to analyze the spectrum-effect correlation,so as to screen out the ingredients with greater contribution to efficacy.Results It was found that 60 batches of samples were clustered into two categories by cluster analysis;A total of 15 differential common peaks were screened by OPLS-DA between the two groups;PLSR analysis suggested that among the 39 identified common peaks,the compounds most relevant to the antithrombotic activity of S.miltiorrhiza might be peak 1(disaccharide),peak 31(neocryptotanshinone),peak 26(tanshialdehyde),peak 34(cryptotanshinone),peak 39(dihydro-crytotanshinone),peak 8(rosmarinic acid),peak 18(4-methyl salvianolate B or isomer)and peak 17(salvianolic acid B).Conclusion This study preliminarily explains the pharmacodynamic material basis of antithrombotic effect of S.miltiorrhiza by means of the spectrum-efficacy of traditional Chinese medicine,which provides scientific basis for the establishment of spectrumeffect related quality evaluation system,and provides data support on the clinical treatment of thrombotic diseases with S.miltiorrhiza.

关 键 词：丹参 UPLC-Q-Exactive-Orbitrap MS 斑马鱼 抗血栓 偏最小二乘回归分析 新隐丹参酮 丹参醛 隐丹参酮 二氢隐丹参酮 迷迭香酸 4-甲基丹酚酸酯B 丹酚酸B 

分 类 号：R285.5[医药卫生—中药学]