基于UPLC-Q-TOF/MS鉴定健脾益肠散大鼠体内外源物成分  

Identification of xenobiotics of Jianpi Yichang Powder in rats based on UPLC-Q-TOF/MS

作　　者：孔心雨 李璐[2,3] 商晶 刘文君[2,3] 钱梦雨 王振中[2,3] 肖伟 KONG Xinyu;LI Lu;SHANG Jing;LIU Wenjun;QIAN Mengyu;WANG Zhenzhong;XIAO Wei(Nanjing University of Chinese Medicine,Nanjing 210000,China;Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China;National Key Laboratory on Technologies for Chinese Medicine Pharmaceutical Process Control and Intelligent Manufacture,Lianyungang 222001,China)

机构地区：[1]南京中医药大学,江苏南京210000 [2]江苏康缘药业股份有限公司,江苏连云港222001 [3]中药过程控制与智能制造技术全国重点实验室,江苏连云港222001

出　　处：《中草药》2024年第6期1908-1924,共17页Chinese Traditional and Herbal Drugs

基　　金：国家中医药管理局“岐黄学者”项目(2021)。

摘　　要：目的基于UPLC-Q-TOF/MS技术研究健脾益肠散在正常大鼠体内的原型成分和代谢产物,归纳总结不同类型化合物的体内代谢规律。方法采用Agilent ZORBAX Eclipse Plus C18(100 mm×2.1 mm,1.8μm)色谱柱,流动相为甲醇(A)-0.1%甲酸水(B),梯度洗脱,体积流量0.4 mL/min,柱温30℃,电喷雾离子源(ESI),在正、负离子模式下对色谱流出物进行质谱检测。结果从大鼠血浆、尿液、粪便中鉴定出34个原型成分、79个代谢成分,主要代谢途径包括氧化、还原、甲基化、葡糖醛酸结合、硫酸结合等。结论对健脾益肠散在大鼠体内的外源物成分进行快速有效的定性,为揭示其功效成分的作用机制提供物质基础。Objective To study the prototypes and their metabolites of Jianpi Yichang Powder(健脾益肠散)in normal rats by UPLC-Q-TOF/MS,and summarize the metabolic rules of different types of compounds in vivo.Methods The separation was performed on Agilent ZORBAX Eclipse Plus C18 column(100 mm×2.1 mm,1.8μm)with methyl alcohol(A)and 0.1%formic acid water(B)as the mobile phase by gradient elution,and the flow rate was 0.4 mL/min,the column temperature was 30°C.The electro-spray ionization(ESI)was applied for the qualitative analysis of chromatographic effluents under the positive and negative ion modes.Results A total of 34 prototypes and 79 metabolites were identified from rat plasma,urine,and feces.The main metabolic pathways mainly included oxidation,reduction,methylation,glucuronic acid binding,sulfuric acid binding and so on.Conclusion The rapid and effective qualitative identification of the xenobiotics of Jianpi Yichang Powder in rats would provide a material basis for revealing the mechanism of action of its functional components.

关 键 词：健脾益肠散 UPLC-Q-TOF/MS 原型成分 代谢产物 补骨脂素 

分 类 号：R284.1[医药卫生—中药学]