鱼用降肝脂益生菌的筛选及鉴定  

Screening and identification of liver lipid-lowering probiotic bacteria for fish

作　　者：刘洪瑞 王涛[2] 孙家塍 张晓月 孙金生[1] 左志晗[1] LIU Hongrui;WANG Tao;SUN Jiacheng;ZHANG Xiaoyue;SUN Jinsheng;ZUO Zhihan(College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin 300387,China)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《天津师范大学学报（自然科学版）》2024年第1期56-61,共6页Journal of Tianjin Normal University：Natural Science Edition

基　　金：天津市人才发展特殊支持计划高层次创新创业团队项目(ITTFRS2017007);天津市高等学校创新团队建设规划项目(TD13-5076).

摘　　要：为了获得具有预防及缓解鱼类脂肪肝作用的益生菌,以从半滑舌鳎肠道中分离出的13株水产动物肠道菌为实验菌株,通过体外产胆盐水解酶能力检测,获得产胆盐水解酶能力较强的菌株.将13株菌株进行斑马鱼的饲喂与浸浴实验,测定斑马鱼的体质量和肝脏中甘油三酯含量并进行肝脏油红O染色切片观察,对于可高效降解斑马鱼肝脏脂肪的菌株进行分子鉴定和系统发育树构建.结果显示,菌株ND-1和U3的产胆盐水解酶能力最强,其次是菌株SC-03和YA6.菌株ND-1和YA6的胆固醇去除率分别为89.76%和89.69%;菌株M4和U3的胆固醇去除率分别为80.26%和76.39%.菌株YZ-02、U2、SC-04和ND-1可以减轻斑马鱼的体质量,菌株SC-01、YZ-02、U3和SH-1可以降低斑马鱼肝脏中的甘油三酯含量,菌株YZ-01、ND-1和U3可有效缓解斑马鱼肝脏中脂滴的沉积.综合来看,菌株U3和ND-1对斑马鱼肝脏脂肪肝的降脂效果最好,分子鉴定和系统发育树构建结果表明,ND-1可与马胃葡萄球菌(Staphylococcus equorum strain Y15,JX134628)聚为一支,置信度为100%,菌株U3能够与鼠李糖乳酸杆菌(Lacticaseibacillus rhamnosus strain 5681,MT463591)聚为一支,置信度为98%.To obtain probiotics that can prevent and alleviate fatty liver disease in fish,13 strains of aquatic animal gut bacteria screened from Gynoglossus semilaevis Gunther were used as experimental strains.The strains with strong bile salt hydrolase production were obtained through the detection of bile salt hydrolase production in vitro.The feeding and bathing experiments of zebrafish were carried out with 13 strains.The body mass and liver triglyceride content of zebrafish were determined,and the liver sections were stained with oil red O for observation.Molecular identification and phylogenetic tree construction were car-ried out for the strains which could degrade zebrafish liver fat efficiently.The results showed that strains ND-1 and U3 had the highest bile salt hydrolase activity,followed by strains SC-03 and YA6.The cholesterol removal rates of strains ND-1 and YA6 were 89.76%�and 89.69%�,and those of strains M4 and U3 were 80.26%�and 76.39%,respectively.Strains YZ-02,U2,SC-04 and ND-1 could reduce the body mass of zebrafish,strains SC-01,YZ-02,U3 and SH-1 reduced the triglyceride content in ze-brafish liver,and strains YZ-01,ND-1 and U3 could effectively alleviate lipid droplets deposition in zebrafish liver.Overall,strains U3 and ND-1 had the best effect on reducing lipid in fatty liver of zebrafish.The results of molecular identification and construction of phylogenetic tree showed that ND-1 could combine with Staphylococcus equiorum strain Y15(JX134628)with confidence of 100%,and strain U3 could combine with Lactobacillus rhamnosus strain 5681(MT463591)with confidence of 98%.

关 键 词：降脂 益生菌 斑马鱼 脂肪肝 水产养殖 

分 类 号：Q939.9[生物学—微生物学]