鹅不食草化学成分及其抗炎活性研究  

Study on Chemical Constituents of Centipeda minima and Anti-inflammatory Activity

作　　者：杨柳[1,2] 高奇 周浪[1,3] 李齐激 杨娟[1,3] 杨艳 YANG Liu;GAO Qi;ZHOU Lang;LI Qi-ji;YANG Juan;YANG Yan(The Key Laboratory of Chemistry for Natural Products of Guizhou Province,Chinese Academy of Sciences,Guiyang 550014,China;College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;State Key Laboratory of Func-tions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550014,China)

机构地区：[1]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014 [2]贵州中医药大学药学院,贵州贵阳550025 [3]贵州医科大学省部共建药用植物功效与利用国家重点实验室,贵州贵阳550014

出　　处：《中药材》2023年第8期1924-1930,共7页Journal of Chinese Medicinal Materials

基　　金：贵州省科技创新能力建设专项(黔科合服企[2020]4013号);贵州省教育厅高校特色重点实验室(黔教合KY字[2020]018号)。

摘　　要：目的:研究鹅不食草的化学成分及其抗炎活性。方法:采用柱色谱和制备液相色谱等方法对鹅不食草的80%乙醇提取物进行分离纯化,结合NMR、MS等现代波谱技术对化合物进行结构鉴定;通过检测化合物对脂多糖(LPS)诱导小鼠巨噬细胞(RAW264.7)释放炎症介质NO评价其抗炎活性。结果:从鹅不食草中共分离纯化得到22个化合物,分别鉴定为:蒲公英甾醇醋酸酯(1)、角鲨烯(2)、羽扇烯酮(3)、蒲公英甾醇(4)、无羁萜(5)、蒲公英甾酮(6)、伪蒲公英甾醇醋酸酯(7)、lupeol acetate(8)、β-谷甾醇(9)、9-羟基百里酚(10)、8-hydroxy-9-isobutyryloxy-10(2)-methylbutyryloxythymol(11)、8,10-dihydroxy-9-isobutyryloxythymol(12)、E-pcoumaryl-alcohol ethyl ether(13)、2-hydroxy-4-methy-lbenzoic acid(14)、3-deuteriomethyl-5-methyl-2,3-dihydrobenzofyran(15)、间苯二甲醚(16)、香草酸(17)、ethyl-2-(3,4-dihydroxyphenyl)acetate(18)、5-羟基-6,8-二甲氧基香豆素(19)、吲唑(20)、糙叶败酱碱(21)、5-(hydroxymethyl)-2,4,4-trimethyl-2-cyclohexen-1-one(22)。结论:其中,化合物2、3、5、6、13~22为首次从石胡荽属植物中分离得到,化合物10~15、17、19~22表现出较强的抗炎活性。Objective:To study the chemical constituents and anti-inflammatory activity of Centipeda minima.Methods:The chemi-cal constituents of the 80%ethanol extract of Centipeda minima were isolated and purified by various column chromatography and pre-parative liquid methods,and the structures were identified on the basis of NMR and MS spectral analysis.The anti-inflammatory activity of the compounds was evaluated by detecting the release of inflammatory mediators NO from mice macrophages(RAW264.7)induced by lipopolysaccharide(LPS).Results:Twenty-two compounds were isolated from Centipeda minima and identified as taraxasteryl acetate(1),squalene(2),lupenone(3),taraxasterol(4),friedelin(5),taraxasterone(6),pseudotaraxasterol acetate(7),lupeol acetate(8),β-sitosterol(9),9-hydroxythymol(10),8-hydroxy-9-isobutyryloxy-10(2)-methylbutyryloxythymol(11),8,10-dihydroxy-9-isobutyryloxy-thymol(12),E-pcoumaryl-alcohol ethyl ether(13),2-hydroxy-4-methy-lbenzoic acid(14),3-deuteriomethyl-5-methyl-2,3-dihydro-benzofyran(15),1,3-dimethoxybenzene(16),vanillic acid(17),ethyl-2-(3,4-dihydroxyphenyl)acetate(18),5-hydroxy-6,8-dime-thoxycoumarin(19),indazole(20),patriscabratine(21),5-(hydroxymethyl)-2,4,4-trimethyl-2-cyclohexen-1-one(22),respectively.Conclusion:Among them,compounds 2,3,5,6,13 to 22 are isolated from Centipeda genus for the first time,compounds 10 to 15,17,19 to 22 show strong anti-inflammatory activity.

关 键 词：鹅不食草 化学成分 抗炎活性 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学] R285.5