两种乔木凋落叶浸提液处理对地毯草土壤酶活性及其化学计量比的影响  

Effect of two kinds of tree litter leaf extracts on soil enzyme activities and eco enzymatic stoichiometry of Axonopus compressus

作　　者：黄琳曦 陈倩[1] 张先言 闫顺 杨云 辛培尧[1] 汪琼[1] HUANG Lin-xi;CHEN Qian;ZHANG Xian-yan;YAN Shun;YANG Yun;XIN Pei-yao;WANG Qiong(College of Landscape Gardening,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南林业大学园林园艺学院,云南昆明650224

出　　处：《草业学报》2024年第4期35-46,共12页Acta Prataculturae Sinica

基　　金：科技人才与平台计划项目(院士专家工作站)(202205AF150022)资助。

摘　　要：乔木搭配人工草坪是园林绿化中常见的植物配置方案,但乔木凋落物可能会影响林下草坪草的生长。为探究两种乔木栾树、樱树凋落物对地毯草草坪的影响,本研究将栾树、樱树凋落叶分别制成10、20、40 g·L^(-1)质量浓度的水浸提液,通过盆栽试验分析两种凋落叶浸提液处理下地毯草根际土壤水解酶活性及其化学计量比的变化,初步探讨林下植被管理对地毯草土壤质量的影响。结果表明:1)在不同质量浓度的栾树凋落叶浸提液处理下,地毯草根际土壤β-1,4-葡萄糖苷酶(BG)、β-N-乙酰氨基葡萄糖苷酶(NAG)、酸性磷酸酶(PHOS)活性均呈先增后减的变化趋势,木糖聚酶(XYL)则呈先减少后增加,最后减少的变化趋势,而在不同质量浓度的樱树凋落叶浸提液处理下,地毯草根际土壤β-1,4-葡萄糖苷酶、β-N-乙酰氨基葡萄糖苷酶、木糖聚酶活性则表现为先减后增的变化规律,酸性磷酸酶随质量浓度的增加呈递减的变化规律;2)相关性分析表明,N、P获取酶活性与土壤有机碳(SOC)含量呈显著正相关,酶C/N、酶C/P与总氮(TN)、总磷(TP)含量呈显著负相关,酶N/P与SOC含量呈显著负相关。矢量模型分析发现凋落物浸提液处理下地毯草根际土壤微生物呈P养分限制特征,樱树凋落叶浸提液处理能缓解微生物C、P限制;3)冗余分析进一步揭示了栾树和樱树凋落叶浸提液处理下地毯草根际土壤SOC、TP含量和土壤C/P、土壤含水量(SWC)、pH是影响土壤酶活性及其化学计量比的主要因子。因此,在人工草坪日常管理中应适时添加栾树、樱树凋落叶,在提高土壤微生物酶活性的同时缓解了地毯草生长中的碳和磷限制。Matching trees with artificial turf is a common plant configuration scheme in landscaping,but tree litter may affect the growth of understory lawn grass.The aim of this study,therefore,was to explore the allelopathic effects of litter leaf extracts of Koelreuteria paniculata and Prunus serrulata on Axonopus compressus lawn as a preliminarily investigation of the impact of understory vegetation management on the soil quality of A.compressus.Aqueous extracts of K.paniculata and P.serrulata leaf litter were prepared at mass concentrations of 10,20,and 40 g·L^(-1),and applied to A.compressus growing in pots.Hydrolase activity and its stoichiometric changes were determined in the rhizosphere soil of A.compressus treated with the two kinds of litter extracts.It was found that:1)As the concentration of the K.paniculata extract increased,the activities ofβ-1,4-glucosidase,β-N-acetylglucosaminidase,and acid phosphatase in the rhizosphere soil of A.compressus initially increased and then decreased,whereas xylase activity initially decreased,then increased,and then decreased.As the concentration of the P.serrulata extract increased,the activities of β-1,4-glucosidase,β-N-acetylglucosaminidase,and xylase in the rhizosphere soil of A.compressus initially decreased and then increased,whereas the activity of acid phosphatase continuously decreased.2)Correlation analyses showed that the activities of nitrogen-and phosphorus-acquisition enzymes were significantly positively correlated with soil organic carbon(SOC)content;Enzyme carbon∶nitrogen(C∶N)and enzyme carbon∶phosphorus(C∶P)were significantly negatively correlated with total nitrogen(TN)and total phosphorus(TP),respectively.And enzyme N∶P was significantly negatively correlated with the SOC content.The results of a vector model analysis revealed that the microorganisms in the rhizosphere soil initially had phosphorus limitation characteristics,but treatment with the P.serrulata litter leaf extract alleviated microbial C and P limitation.3)A redundancy analysi

关 键 词：凋落叶 土壤酶活性 化学计量比 养分限制 

分 类 号：S812.2[农业科学—草业科学]