紫花苜蓿SAUR基因家族的鉴定及其在非生物胁迫中的表达模式研究  

Identification of the alfalfa SAUR gene family and its expression pattern under abiotic stress

作　　者：刘昊 李显炀 何飞 王雪 李明娜[1] 龙瑞才[1] 康俊梅[1] 杨青川[1] 陈林 LIU Hao;LI Xian-yang;HE Fei;WANG Xue;LI Ming-na;LONG Rui-cai;KANG Jun-mei;YANG Qing-chuan;CHEN Lin(Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Grassland Science,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]青岛农业大学草业学院,山东青岛266109

出　　处：《草业学报》2024年第4期135-153,共19页Acta Prataculturae Sinica

基　　金：中央及公益性科研院所基本科研业务费专项(2022-YWF-ZYSQ-04);内蒙古自治区种业科技创新重大示范工程“揭榜挂帅”项目(2022JBGS0016)资助。

摘　　要：SAUR(small auxin-up RNA)是植物早期响应生长素的一类基因,参与植物生长发育与非生物胁迫响应等一系列生物过程。在拟南芥、水稻、棉花等物种中已经对SAUR基因家族进行了系统鉴定与分析,但是,在世界上最重要的豆科牧草紫花苜蓿中关于SAUR基因家族的研究尚未开展。本研究利用生物信息学的方法在紫花苜蓿参考基因组共鉴定到433个MsSAUR成员,并对其基因组位置、基因结构、启动子顺式作用元件以及不同组织中的表达模式进行了分析。同时,利用紫花苜蓿在不同非生物胁迫下的转录组数据,发现有5、11、19以及12个MsSAUR成员分别响应干旱、盐、冷以及碱胁迫,MsSAUR14/94/254可以同时响应干旱和盐胁迫,MsSAUR297可以同时响应干旱、盐以及碱胁迫,MsSAUR306可以同时响应干旱、盐以及冷胁迫。本研究结果可为后期通过基因工程技术创制高产抗逆新种质提供重要的候选基因。SAUR(small auxin up-regulated RNA)is a category of auxin gene in plant early response,involved in a series of biological processes such as plant growth and development and abiotic stress response.The SAUR gene family has been systematically identified and analyzed in Arabidopsis thaliana,rice(Oryza sativa),cotton(Gossypium spp.)and other species,but the research on the SAUR gene family in alfalfa(Medicago sativa),the most important leguminous forage in the world,has not been carried out.In this study,a total of 433 MsSAUR gene family members were identified in the reference genome of alfalfa using bioinformatics methods,and their genome location,gene structure,promoter cis-acting elements and expression patterns in different tissues were analyzed.Meanwhile,using transcriptome data of alfalfa under different abiotic stresses,it was found that five MsSAUR gene family members responded to drought stress,11 responded to salt,19 responded to cold and 12 responded to alkali stress.MsSAUR14/94/254 could respond to drought and salt stress simultaneously,and MsSAUR297 could respond to drought,salt and alkali stress simultaneously.MsSAUR306 can respond to drought,salt and cold stress simultaneously.The results of this study can provide important candidate genes for the creation,using genetic engineering,of new germplasm with high yield and resistance.

关 键 词：紫花苜蓿 SAUR基因 全基因组鉴定 非生物胁迫 表达模式 

分 类 号：S541.9[农业科学—作物学]