全基因组水平紫花苜蓿WRKY转录因子家族鉴定与表达模式分析  

Identification and expression pattern of the WRKY transcription factor family in Medicago sativa

作　　者：李显炀 刘昊 何飞 王雪 李明娜[1] 龙瑞才[1] 康俊梅[1] 杨青川[1] 陈林 LI Xian-yang;LIU Hao;HE Fei;WANG Xue;LI Ming-na;LONG Rui-cai;KANG Jun-mei;YANG Qing-chuan;CHEN Lin(Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Life Science and Technology,Harbin Normal University,Harbin 150025,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨150025

出　　处：《草业学报》2024年第4期154-170,共17页Acta Prataculturae Sinica

基　　金：中央及公益性科研院所基本科研业务费专项(2022-YWF-ZYSQ-04);内蒙古自治区种业科技创新重大示范工程“揭榜挂帅”项目(2022JBGS0016)资助。

摘　　要：WRKY转录因子是植物中最大的基因家族之一,在植物生长发育以及非生物胁迫过程中发挥重要的作用。目前,WRKY基因家族成员已经在多个植物物种中进行了广泛的研究和分析。然而,WRKY转录因子尚未在四倍体紫花苜蓿全基因组水平上进行鉴定与分析。本研究系统鉴定了紫花苜蓿WRKY基因家族成员,并对其在不同胁迫处理下的表达模式进行了分析。结果显示,共有198个WRKY转录因子不均匀分布在紫花苜蓿的32条染色体上,其中7号染色体的数量最多。系统进化树结果显示,WRKY基因家族成员可以分为3个组。在这些WRKY转录因子中,有42个响应盐胁迫,7个响应碱胁迫,19个响应冷胁迫和37个响应干旱胁迫,同时发现MsWRKY154和MsWRKY166可以同时响应盐、干旱、冷以及碱胁迫。本研究的结果可为紫花苜蓿WRKY转录因子的生物学功能深入研究奠定基础,同时可为紫花苜蓿分子育种提供有价值的信息。WRKY transcription factors are one of the largest gene families in plants and play important roles in plant growth and development as well as in abiotic stress processes.Currently,members of the WRKY gene family have been extensively studied and analyzed in a variety of plant species.However,the alfalfa(Medicago sativa)WRKY transcription factors have not been identified and analyzed at the genome-wide level.In this study,we systematically identified alfalfa WRKY gene family members and analyzed their expression patterns under different stress treatments.We showed that a total of 198 WRKY transcription factors were unevenly distributed across 32 chromosomes in alfalfa,with chromosome 7 having the largest number.The phylogenetic tree results show that the WRKY gene family members can be divided into three groups.Among these WRKY transcription factors,42 responded to salt stress,7 to alkali stress,19 to cold stress and 37 to drought stress,and MsWRKY154 and MsWRKY166 were found to respond to both salt,drought,and cold as well as alkali stress.The results of this study provide a foundation for the intensive investigation of the biological function of WRKY transcription factors in alfalfa,and can also provide valuable information for molecular breeding in alfalfa.

关 键 词：WRKY转录因子 紫花苜蓿 表达模式 非生物胁迫 

分 类 号：S541.9[农业科学—作物学]