长链非编码RNA-NRON对小鼠心肌梗死后细胞凋亡的作用机制研究  

Effects of long noncoding RNA-NRON on apoptosis following myocardial infarction in mice

作　　者：高涵 张春晶 李淑艳 师岩 郭红艳 杨超 GAO Han;ZHANG Chunjing;LI Shuyan;SHI Yan;GUO Hongyan;YANG Chao(Department of Biochemistry,College of Medical Technology,Qiqihar Medical University,Qiqihar161006,China)

机构地区：[1]齐齐哈尔医学院医学技术学院生物化学教研室,黑龙江齐齐哈尔161006

出　　处：《中国医科大学学报》2024年第3期218-223,共6页Journal of China Medical University

基　　金：黑龙江省省属高等学校基本科研业务费项目(2023-KYYWF-0860);黑龙江省卫生健康委医药卫生科研课题(20210202020361);齐齐哈尔医学科学院项目(QMSI2020B-04);齐齐哈尔医学科学院重点培育项目(2022-ZDPY-011);齐齐哈尔市科技计划联合引导项目(LSFGG-2022057)。

摘　　要：目的探讨长链非编码RNA(lncRNA)-NRON对小鼠心肌梗死(MI)后细胞凋亡的影响。方法将C57BL/6小鼠随机分为假手术(Sham)组、MI组、MI合并注入lncRNA-NRON干扰慢病毒(MI+shNRON)组和MI合并注入NC阴性对照慢病毒(MI+NC)组。采用实时PCR检测lncRNA-NRON的表达;用HE染色、TTC染色、TUNEL染色检测心肌组织病理损伤、MI面积、细胞凋亡情况;用RPIseq数据库预测lncRNA-NRON与电压依赖性阴离子通道蛋白(VDAC)的互作概率;用Western blotting检测lncRNA-NRON对VDAC蛋白表达的影响。结果lncRNA-NRON在MI组表达显著升高,特异性敲减lncRNA-NRON后,可减轻心肌组织病理损伤,减少MI面积,抑制细胞凋亡,lncRNA-NRON与VDAC的互作概率高达0.9,且lncRNA-NRON可调控VDAC的蛋白表达。结论敲减lncRNA-NRON可抑制MI后心肌损伤的发生,其机制可能是通过lncRNA-NRON与VDAC结合并抑制其表达,从而影响细胞凋亡进程。Objective To investigate the effects of long noncoding RNA(lncRNA)-NRON on apoptosis following myocardial infarc-tion(MI)in mice.Methods The C57BL/6 mice were randomly divided into four groups:sham operation(Sham)group,MI group,MI combined with lncRNA-NRON interference lentivirus(MI+shNRON)group,and MI combined with the negative control(NC)lentivirus(MI+NC)group.The expression of lncRNA-NRON was detected using real-time PCR.In addition,the pathology of the myocardial tissue injury was analyzed using HE staining,the myocardial infarction size was examined using TTC staining,and the extent of apoptosis was assessed using the TUNEL assay,respectively.The RPISeq database was used to predict the probability of interaction between lncR-NA-NRON and the voltage-dependent anionic channel protein(VDAC).The effect of lncRNA-NRON on the expression of VDAC protein was detected using Western blotting.Results The lncRNA-NRON expression was significantly increased in the MI group,and the tar-geted knockdown of lncRNA-NRON resulted in alleviation of the pathological myocardial tissue injury,reduction in the myocardial infarc-tion area,and inhibition of apoptosis.The probability of interaction between lncRNA-NRON and VDAC reached 0.9,indicating a high probability of their association.Additionally,lncRNA-NRON could regulate the protein expression of VDAC.Conclusion Knockdown of lncRNA-NRON could reduce the occurrence of myocardial injury following myocardial infarction.This effect may be attributable to a spe-cific mechanism wherein lncRNA-NRON affects the process of apoptosis by binding to VDAC,consequently suppressing its expression.

关 键 词：长链非编码RNA-NRON 心肌梗死 电压依赖性阴离子通道蛋白 细胞凋亡 

分 类 号：R542.2[医药卫生—心血管疾病]