胎儿生长受限孕妇胎盘自噬相关蛋白表达水平及其临床意义  

Expression levels and clinical significance of autophagy related proteins in placenta tissues of pregnant women with fetal growth restriction

作　　者：张玉苓 张珂[1] 许艇 张庆[1] ZHANG Yuling;ZHANG Ke;XU Ting;ZHANG Qing(Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China)

机构地区：[1]郑州大学第二附属医院妇产科,河南郑州450014

出　　处：《基础医学与临床》2024年第4期528-532,共5页Basic and Clinical Medicine

基　　金：河南省医学科技攻关计划项目(LHGJ20230334)。

摘　　要：目的 探讨胎儿生长受限(FGR)孕妇胎盘自噬相关蛋白Beclin-1、微管相关蛋白轻链3(LC3)表达水平及其临床意义。方法 选取2022年08月至2023年08月于郑州大学第二附属医院因FGR分娩的孕妇胎盘组织40例作为研究对象,另选取同期正常分娩的孕妇胎盘组织40例为健康组。收集两组一般临床资料,RT-qPCR法检测胎盘组织中Beclin-1、LC3 mRNA,免疫组化法检测胎盘组织Beclin-1、LC3蛋白表达,分析不同临床资料FGR胎盘组织Beclin-1、LC3蛋白阳性率差异。结果 FGR组胎盘厚度、胎盘质量和新生儿体质量均低于健康组(P<0.05)。FGR组Beclin-1 mRNA、LC3 mRNA水平均高于健康组(P<0.05)。FGR组自噬相关蛋白Beclin-1、LC3阳性表达率均显著高于健康组(P<0.05)。FGR胎盘组织Beclin-1、LC3蛋白阳性表达率在胎盘厚度、质量和新生儿体质量上均有差异(P<0.05)。结论 自噬基因Beclin-1和LC3的阳性表达情况与FGR有关。Objective To explore the expression and clinical significance of autophagy related proteins Beclin-1,microtubule-associated protein 1 light chain 3(LC3)in placenta tissues of pregnant women with fetal growth restriction(FGR).Methods A total of 40 pregnant women undergoing delivery due to FGR and 40 pregnant women undergoing normal delivery in the Second Affiliated Hospital of Zhengzhou University were enrolled as FGR group and healthy group between August 2022 and August 2023.The general clinical data in the two groups were collected.The levels of Beclin-1 and LC3 mRNA in placenta tissues were detected by PCR,and expressions of Beclin-1 and LC3 proteins were detected by immunohistochemistry.The differences in positive of Beclin-1 and LC3 proteins among patients with different clinical data were analyzed.Results The placental thickness,placental mass and neonatal weight in FGR group were lower than those in healthy group(P<0.05).The mRNA levels of Beclin-1 and LC3 in FGR group were higher than those in healthy group(P<0.05)and positive expression rates of Beclin-1 and LC3 proteins were significantly higher than those in healthy group(P<0.05).There was significant difference in positive expression profile of Beclin-1 and LC3 proteins among patients with different placental thickness,placental mass and neonatal weight(P<0.05).Conclusions The positive expressions of autophagy genes(Beclin-1 and LC3)are related to FGR,and their specific expression levels are closely related to fetal growth and development.

关 键 词：胎儿生长受限 自噬相关蛋白 相关性 临床特征 

分 类 号：R714.5[医药卫生—妇产科学]