不同产地滇黄精高效液相色谱法指纹图谱的建立及其多糖含量测定  

Establishment of high performance liquid chromatography fingerprint and determination of polysaccharide content in Polygonatum kingianum from different origins

作　　者：马琴 郭思潍 杜强 杨国海 王才武 周雪 吴林菁 MA Qin;GUO Siwei;DU Qiang;YANG Guohai;WANG Caiwu;ZHOU Xue;WU Linjing(Key Laboratory of Optimal Utilization of Natural Medicine Resources&the High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Druggability,School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 561113,Guizhou,China;Guizhou Academy of Agricultural Sciences,Guiyang 550025,Guizhou,China;Tianzhu Sanfangtian Planting Cooperative,Tianzhu 556608,Guizhou,China)

机构地区：[1]贵州医科大学药学院天然药物资源优效利用重点实验室&贵州省高等学校天然药理与成药性评价重点实验室,贵州贵阳561113 [2]贵州省农业科学院,贵州贵阳550025 [3]天柱县三方田种植专业合作社,贵州天柱556608

出　　处：《贵州医科大学学报》2024年第3期381-388,共8页Journal of Guizhou Medical University

基　　金：贵州省科技计划项目(黔科合基础-ZK〔2022〕一般389,黔科合支撑〔2020〕4Y104,黔科合支撑〔2023〕一般046);贵州省科技创新基地(黔科合中引地〔2023〕003);贵州省高层次创新型人才百层次人才(黔科合平台人才-GCC〔2023〕048)。

摘　　要：目的探讨不同产地滇黄精高效液相色谱法(HPLC)指纹图谱的建立及其多糖含量的测定。方法取贵州(剑河、安顺、天柱、铜仁、赤水、贵阳及纳雍)、广西百色及四川内江来源的9个批次滇黄精药材各1 g制备供试品溶液,采用DiKMA Platisll ODS C_(18)色谱柱(流动相为乙腈-0.05%磷酸水溶液、梯度洗脱,流速1 mL/min,柱温35℃,进样量20μL,检测波长203 nm)建立滇黄精HPLC指纹图谱,并采用相似度评价、主成分分析及聚类分析等方法评价不同产地滇黄精的质量;采用蒽酮-硫酸法测定各批次滇黄精的多糖含量。结果建立了9批滇黄精的HPLC指纹图谱,共确认12个共有峰;9批不同产地的滇黄精多糖含量均大于7%,含量顺序为贵州剑河>贵州安顺>贵州天柱>贵州铜仁>贵州赤水>贵州贵阳>贵州纳雍>广西百色>四川内江;相似度结果显示,四川内江、贵州贵阳及广西百色的相似度分别为0.762、0.809及0.846,贵州其余地区滇黄精的相似度≥0.853;主成分分析结果显示共得到3个主成分因子,聚类分析结果表明滇黄精被分为3类,且滇黄精多糖含量≥7.16%。结论本研究建立的滇黄精HPLC指纹图谱及黄精多糖成分含量的测定方法简便可行,准确可靠。Objective To investigate establishment of high performance liquid chromatography(HPLC)fingerprints and determination of polysaccharides content in Polygonatum kingianum from different origins.Methods Nine batches of Polygonatum kingianum(1 g)from Guizhou(Jianhe,Anshun,Tianzhu,Tongren,Chishui,Guiyang,and Nayong),Baise Guangxi,and Neijiang Sichuan sources were taken to prepare the test solution(1 g from each origin).The separation was performed on a DiKMA Platisll ODS C_(18)(250 mm×4.6 mm,5μm)column(with acetonitrile-0.05%phosphoric acid aqueous solution as mobile phase in gradient elution at a flow rate of 1 mL/min and a column temperature of 35℃ with an injection volume of 20μL and a detection wavelength of 203 nm)to establish HPLC fingerprints of Polygonatum kingianum extracts;the quality of Polygonatum kingianum from different origins was evaluated by similarity evaluation,principal component analysis and cluster analysis;the content of polysaccharides of Polygonatum kingianum from each batch was determined by anthrone-sulfuric acid method.Results HPLC fingerprints were established for nine batches of Polygonatum kingianum,and a total of 12 common peaks were identified.The polysaccharide content in all nine batches from different sources exceeded 7%.Among them,the descending order of polysaccharide content was as follows:Jianhe Guizhou>Anshun Guizhou>Tianzhu Guizhou>Tongren Guizhou>Chishui Guizhou>Nayong Guizho u>Baise Guangxi>Neijiang Sichuan.The similarity analysis results indicated that the similarities between Neijiang Sichuan,Guiyang Guizhou and Baise Guangxi were 0.762,0.809,and 0.846,respectively;while the similarity of Polygonatum kingianum between other areas in Guizhou province were≥0.853.Furthermore,three principal component factors were obtained using principal component analysis.Cluster analysis revealed that Polygonatum kingianum could be categorized into three distinct groups and content of polysaccharides of Polygonatum kingianum were≥7.16%.Conclusion The method of HPLC fingerprints and

关 键 词：聚类分析 主成分分析 滇黄精 指纹图谱 高效液相色谱法 紫外分光光度法 

分 类 号：R282.71[医药卫生—中药学]