基于气相色谱质谱联用技术分析木香及川木香不同炮制品挥发油的化学成分  

Chemical Composition of Volatile Oils from Different Processed Products of Aucklandia Lappa Decne.and Vladimiria Souliei (Franch.) Ling Based on Gaschromatography-Mass Spectrometry

作　　者：梁淞婷 陈瑞鑫 次仁卓嘎 蒋桂华[1,3] 杜蕾蕾 Liang Songting;Chen Ruixin;Ciren Zhuoga;Jiang Guihua;Du Leilei(School of Pharmacy/State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China;School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China;School of Chinese Classics,Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China)

机构地区：[1]成都中医药大学药学院/西南特色中药资源国家重点实验室,四川成都611137 [2]成都中医药大学民族医药学院,四川成都611137 [3]成都中医药大学国学院,四川成都611137

出　　处：《成都中医药大学学报》2024年第2期1-10,共10页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82173928);四川省应用基础研究计划项目(2020YJ0375);四川省中药研究所基础研究项目(2020JDKY0013);四川省中医药管理局项目(2021MS022、2021MS017)。

摘　　要：目的:探讨不同炮制方法对木香及川木香挥发油成分的影响。方法:采用切制、清炒、麸炒、纸煨方法炮制木香与川木香,测定不同炮制品的挥发油含量,应用气相色谱质谱联用技术检测各样品成分组成。结果:经炮制后,药材中挥发油含量减少,且随辅料的加入,油量变更少。鉴定出化合物木香生品38种、清炒品36种、麸炒品34种、纸煨品34种;川木香生品32种、清炒品35种、麸炒品35种、纸煨品34种。木香经过炮制后,水芹烯、桧烯、石竹烯等一些芳香成分含量降低,去氢木香内酯、石竹素、香叶基丙酮等药效成分含量逐渐升高。川木香成分变化与木香相似,但芳香成分更少。木香与川木香的相同成分有去氢木香内酯、单紫杉烯、榄香烯、木香醇、α-木香醇;经正交偏最小二乘法判别分析得木香与川木香的含量差异物质有二氢去氢木香内酯、mokkolactone、α-木香醇、γ-木香醇、α-bergamotenol、萘、单紫杉烯、α-selinene、2-isopropenyl-4a,8-dimethyl-1,2,3,4,4a,5,6,7-octahydronaphthalene、(3Z,3aR,7aS)-3-ethylidene-1,2,3a,4,5,6,7,7a-octahydroindene、榄香烯。结论:木香与川木香挥发油中约60%的化学成分基本相同;与生品相比,炮制后二者挥发油的化学成分均有变化,但二者成分的差异减少。其中纸煨法对木香及川木香的化学成分影响最大,辛香气味化合物的减少与一些药效成分的增多可能是纸煨品实肠止泻的物质基础。Objective:To explore the effect of different processing methods on the volatile oil components of Aucklandia lappa Decne.and Vladimiria souliei(Franch.) Ling.Methods:We use cutting,stir-frying,bran-frying and straw paper-simmering methods to prepare the Aucklandia lappa Decne.and Vladimiria souliei(Franch.) Ling,determine the volatile oil content of different preparations.The volatile oil components were studied usinggaschromatography-mass spectrometry.Results:Thirty-eight compounds were identified in the Aucklandia lappa Decne.,while 36,34,and 34 compounds were identified in the stir-fried,bran-fried,and straw paper-wrapped products,respectively.For Vladimiria souliei(Franch.) Ling,32 compounds were identified in the raw product,while 35,35,and 34 compounds were identified in the stir-fried,bran-fried,and straw paper-wrapped products,respectively.After the processing of Aucklandia lappa Decne.,the content of some aroma components such as α-phellandrene,sabinene,and caryophyllene decreased,and the content of medicinal components such as dehydrocostus lactone,caryophyllene oxide and geranylacetone gradually increased.The variation of components of Vladimiria souliei(Franch.) Ling was similar to that of Aucklandia lappa Decne.,but the aroma components were less.The same components of Aucklandia lappa Decne.and Vladimiria souliei(Franch.) Ling include dehydrocostus lactone,(Z,Z,Z)-heptadeca-1,8,11,14-tetraene,elemene,costol,α-costol.The content difference compounds obtained by orthogonal partial least squares-discriminant analysis included dihydrodehydrocostus lactone,mokkolactone,α-costol,γ-costol,α-bergamotenol,naphthalene,(Z,Z,Z)-heptadeca-1,8,11,14-tetraene,α-selinene,2-isopropenyl-4a,8-dimethyl-1,2,3,4,4a,5,6,7-octahydronaphthalene,(3Z,3aR,7aS)-3-ethylidene-1,2,3a,4,5,6,7,7a-octahydroindene and elemene.Conclusion:About 60% of the components in the volatile oil of Aucklandia lappa Decne.and Vladimiria souliei(Franch.) Ling were approximately same.There were differences in components between Aucklandia lapp

关 键 词：木香 川木香 气相色谱质谱联用法 正交偏最小二乘法判别分析 聚类分析 

分 类 号：R283[医药卫生—中药学] R284.1[医药卫生—中医学]