巨型先天性黑色素细胞痣人源性异种移植模型的建立方法探究  

An investigation of the methodology for establishing a human-derived xenograft model of giant congenital melanocytic nevus

作　　者：解永怡 马欣[1,2] 曾俊文 许小向 邓丹 刘业强 XIE Yongyi;MA Xin;ZENG Junwen;XU Xiaoxiang;DENG Dan;LIU Yeqiang(Shanghai Skin Disease Hospital of Tongji University,Shanghai 200443,China;Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;School of Public Health,Nanchang University,Nanchang 330031,Jiangxi Province,China;Department of Dermatology,Shanghai Children's Medical Center,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China)

机构地区：[1]同济大学附属皮肤病医院,上海200443 [2]上海中医药大学附属岳阳中西医结合医院,上海200437 [3]南昌大学公共卫生学院,南昌330031 [4]上海交通大学医学院附属上海儿童医学中心皮肤科,上海200127

出　　处：《世界临床药物》2024年第2期133-138,共6页World Clinical Drug

基　　金：国家自然科学基金(82173396);上海市“科技创新行动计划”自然科学基金项目(23ZR1456100)。

摘　　要：目的探究巨型先天性黑色素细胞痣(giant congenital melanocytic nevus,GCMN)人源性异种移植模型的建立方法。方法将GCMN患者的皮肤标本移植到2只重度联合免疫缺陷小鼠的背部,加压包扎4周后拆开,观察移植GCMN皮肤的存活情况及组织病理学特征。结果1号小鼠术后1周时死亡,可能与移植面积过大有关。2号小鼠长期存活且移植部位皮肤稳定存活至少28周,移植皮肤与小鼠正常皮肤交界处无瘢痕形成。移植GCMN部位苏木精-伊红染色可见真皮内有上皮样痣细胞及黑素颗粒;免疫组化示黑色素抗原、小眼畸形相关转录因子、S-100蛋白及性别决定区盒基因10均为阳性。结论采用该方法构建的GCMN人源性异种移植动物模型可维持GCMN组织学特征及重要分子表型,移植皮肤长期存活,可用于后续基础实验的开展,有利于对GCMN发病机制及潜在药物研发的进一步探究。Objective To explore the establishment of a human-derived xenograft mouse model for giant congenital melanocytic nevus(GCMN).Methods Skin from GCMN patients was transplanted onto the backs of two mice with severe combined immunodeficiency,which were then removed after 4 weeks of pressure bandaging.The survival and histopathological characteristics of the transplanted GCMN skin were observed.Results Mouse No.1 died at 1 week after operation,which may be related to overlarge graft area.Mouse No.2 survived for a long time.The skin xenograft was stable for at least 28 weeks.There was no scar formation at the junction of grafted skin and normal mouse skin.HE staining at GCMN site showed that there were epithelioid nevus cells and melanin granules in the dermis,and immunohistochemistry showed that melanin antigen,microphthalmia transcription factor,S-100 protein,Sry-related HMG-BOX gene 10 were all positive.Conclusion The human-derived xenograft mouse model of GCMN constructed by this method can maintain the histological features and important molecular phenotypes of GCMN.Meanwhile,the transplanted skin can survive for a long time,which can be used for subsequent basic experiments,and is conducive to the further investigation of the pathogenesis of GCMN and its potential drug development.

关 键 词：巨型先天性黑色素细胞痣 动物模型 皮肤移植 

分 类 号：R758.51[医药卫生—皮肤病学与性病学] R-33[医药卫生—临床医学]