Design of a Novel Electrochemical Aptasensor Based on Molybdenum Disulfide Nanosheets for Lysozyme Detection  

作　　者：Tavoos Beiki Ghasem Najafpour-Darzi Maedeh Mohammadi Mohsen Shakeri 

机构地区：[1]Biotechnology Research Laboratory,Department of Chemical Engineering,Babol Noshirvani University of Technology,Babol 47148-71167,Iran [2]Department of Mechanical Engineering,Babol Noshirvani University of Technology,Babol 47148-71167,Iran

出　　处：《Journal of Analysis and Testing》2024年第1期16-27,共12页分析检测（英文）

基　　金：supported by Babol Noshirvani University of Technology[grant number BNUT/965115004/1402];Iran National Science Foundation(INSF)under grant number 98020065.

摘　　要：The precise detection of lysozyme plays a crucial role in early diagnosis of kidney failure in leukemia patients.Herein,we designed an electrochemical aptasensor to detect lysozyme using a glassy carbon electrode(GCE)modified with molybdenum disulfide(MoS_(2))nanosheets decorated with chitosan(Ch)and gold nanoparticles(Au).In the next steps of aptasensor preparation,aptamer(Apt)strands were immobilized on MoS_(2)-Au-Ch/GCE surface via electrostatic interactions,and bovine serum albumin(BSA)solution was applied to cover unbonded areas on Apt/MoS_(2)-Au-Ch/GCE.The MoS_(2)-Au-Ch nanocomposite can warrant enhanced immobilization of aptamers and strong signals because of wrinkle-like structure and base planes in MoS nanosheets,presence of abundant amino and carboxyl functional groups in chitosan and Au nanoparticles.Differential pulse voltammetry(DPV)was used to evaluate the electrochemical behavior of the aptasensor at each preparation step and its detection performance.The developed electrochemical aptasensor based on MoS nanosheets showed remarkable performance for lysozyme detection in the range from 1 pmol/L to 10 nmol/L with a limit of detection of 2.2 fmol/L.The proposed aptasensor provided excellent selectivity against BSA,uric acid,cytochrome C,lysozyme and their mixture.In addition,it displayed great stability of about 99.58%of the initial signal upon 21-day storage.Tests on human serum and urine samples yielded promising recoveries ranging from 98 to 105%.Hence,MoS_(2)-Au-Ch nanocomposite-based aptasensor could be an outstanding candidate for medical device applications and protein quantification.

关 键 词：Electrochemical aptasensor LYSOZYME MoS_(2)nanosheets Apt/MoS_(2)-Au-Ch/GCE 

分 类 号：R318[医药卫生—生物医学工程] O657.1[医药卫生—基础医学]