A strategy to enhance the insecticidal potency of Vip3Aa by introducing additional cleavage sites to increase its proteolytic activation efficiency  

作　　者：Kun Jiang Zhe Chen Yiting Shi Yuanrong Zang Chengbin Shang Xi Huang Jiahe Zang Zhudong Bai Xuyao Jiao Jun Cai Xiang Gao 

机构地区：[1]State Key Laboratory of Microbial Technology,Shandong University,Qingdao 266237,China [2]School of Life Sciences,Shandong University,Qingdao 266237,China [3]Taishan College,Shandong University,Jinan 250100,China [4]Department of Microbiology,College of Life Sciences,Nankai University,Tianjin 300071,China

出　　处：《Engineering Microbiology》2023年第4期3-10,共8页工程微生物学（英文）

基　　金：supported by the National Natural Science Foundation of China(3190194332122007,and 32272610);the Shandong Provincial Natural Science Foundation(ZR2021JQ09);the Major Basic Program of Natural Science Foundation of Shandong Province(ZR2019ZD21);the Key R&D Program of Shandong Province(2020CXGC011305);the Youth Interdisciplinary Innovative Research Group of Shandong Univer-sity(2020QNQT009);the China Postdoctoral Science Foundation funded project(2019T120585 and 2019M652370).

摘　　要：Microbially derived,protein-based biopesticides have become a vital element in pest management strategies.Vip3 family proteins from Bacillus thuringiensis have distinct characteristics from known insecticidal Cry toxins and show efficient insecticidal activity against several detrimental lepidopteran pests.They are considered to be a promising toxic candidate for the management of various detrimental pests.In this study,we found that in addition to the preliminary digestion sites lysine,there are multiple cleavage activation sites in the linker region between domain I(DI)and DII of Vip3Aa.We further demonstrated that by adding more cleavage sites between DI and DII of Vip3Aa,its proteolysis efficiency by midgut proteases can be significantly increased,and correspondingly enhance its insecticidal activity against Spodoptera frugiperda and Helicoverpa armigera larvae.Our study promotes the understanding of the insecticidal mechanism of Vip3 proteins and illustrates an eas-ily implementable strategy to increase the insecticidal potency of Vip3Aa.This facilitates their potential future development and efficient application for sustainable agriculture.

关 键 词：Insecticidal protein Activation mechanism Insecticidal activity modification Insecticidal mechanism Bacillus thuringiensis 

分 类 号：Q93[生物学—微生物学]