Investigating the cellular functions of β-Glucosidases for synthesis of lignocellulose-degrading enzymes in Trichoderma reesei  

作　　者：Ai-Ping Pang Haiyan Wang Yongsheng Luo Funing Zhang Fu-Gen Wu Zhihua Zhou Zuhong Lu Fengming Lin 

机构地区：[1]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing,China [2]Key Laboratory of Synthetic Biology,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai,China

出　　处：《Engineering Microbiology》2023年第4期48-58,共11页工程微生物学（英文）

基　　金：supported by grants from the National Natural Sci-ence Foundation of China(32170072 and 32200037);the State Key Laboratory of Microbial Technology Open Projects Fund(Project NO M2022-12).

摘　　要：β-glucosidases play an important role in the synthesis of cellulase in fungi,but their molecular functions and mechanisms remain unknown.We found that the 10 putativeβ-glucosidases investigated in Trichoderma ree-sei facilitate cellulase production,with cel3j being the most crucial.Transcriptional analysis revealed that the most affected biological processes in△cel3j strain were cellulase synthesis,ribosome biogenesis,and RNA poly-merases.Moreover,CEL3J was unconventionally transported through the endoplasmic reticulum,bypassing the Golgi apparatus,whereas cel3j overexpression altered cellulase secretion from conventional to unconventional,likely owing to the activated unconventional protein secretion pathway(UPS),as indicated by the upregulation of genes related to UPS.The mTORC1-GRASP55 signaling axis may modulate the unconventional secretion of CEL3J and cellulase.The transcriptional levels of genes associated with DNA replication,the cell cycle,and meiosis were noticeably affected by overexpressing cel3j.These data give new clues for exploring the roles ofβ-glucosidases and the molecular mechanisms of their unconventional secretion in fungi.

关 键 词：Β-GLUCOSIDASE cel3j Unconventional secretion pathway Endoplasmic reticulum GOLGI 

分 类 号：Q55[生物学—生物化学]