Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives  

作　　者：Yangyi Qiao Wenyue Ma Shangjie Zhang Feng Guo Kang Liu Yujia Jiang Yanxia Wang Fengxue Xin Wenming Zhang Min Jiang 

机构地区：[1]State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing,211800,PR China [2]College of Food Science and Light Industry,Nanjing Tech University,Nanjing,211800,PR China

出　　处：《Synthetic and Systems Biotechnology》2023年第4期578-583,共6页合成和系统生物技术（英文）

基　　金：supported by National Key R&D Program of China(2022YFC2105900);the National Natural Science Foundation of China(22078151,22378199,21978130);Jiangsu Natural Science Fund for Distinguished Young Scholars(BK20220052)the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture(XTD2215);Young Elite Scientist Sponsorship Program by CAST(YESS20200174).

摘　　要：Artificial multi-enzyme cascades bear great potential for bioconversion of C1 compounds to value-added chemicals.Over the past decade,massive efforts have been devoted to constructing multi-enzyme cascades to produce glycolic acid,rare functional sugars and even starch from C1 compounds.However,in contrast to traditional fermentation utilizing C1 compounds with the expectation of competitive economic performance in future industrialization,multi-enzyme cascades systems in the proof-of-concept phase are facing the challenges of upscaling.Here,we offered an overview of the recent advances in the construction of in vitro multi-enzyme cascades and whole-cell transformation using C1 compounds as substrate.In addition,the existing challenges and possible solutions were also discussed aiming to combine the strengths of in vitro and in vivo multi-enzyme cascades systems for upscaling.

关 键 词：SCALING PROOF utilizing 

分 类 号：Q814[生物学—生物工程]