Synergetic engineering of Escherichia coli for efficient production of L-tyrosine  

作　　者：Jurong Ping Lian Wang Zhijie Qin Zhemin Zhou Jingwen Zhou 

机构地区：[1]Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology,Jiangnan University,1800 Lihu Road,Wuxi,Jiangsu,214122,China [2]Science Center for Future Foods,Jiangnan University,1800 Lihu Road,Wuxi,Jiangsu,214122,China [3]Key Laboratory of Industrial Biotechnology,Ministry of Education and School of Biotechnology,Jiangnan University,1800 Lihu Road,Wuxi,Jiangsu,214122,China [4]Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology,Jiangnan University,Wuxi,214122,China

出　　处：《Synthetic and Systems Biotechnology》2023年第4期724-731,共8页合成和系统生物技术（英文）

基　　金：supported by the Foundation for Innovative Research Groups of the National Natural Science Foundation of China(32021005).

摘　　要：L-Tyrosine,an aromatic non-essential amino acid,is the raw material for many important chemical products,including levodopa,resveratrol,and hydroxytyrosol.It is widely used in the food,drug,and chemical industries.There are many studies on the synthesis of L-tyrosine by microorganisms,however,the low titer of L-tyrosine limited the industrial large-scale production.In order to enhance L-tyrosine production in Escherichia coli,the expression of key enzymes in the shikimate pathway was up-or down-regulated.The L-tyrosine transport system and the acetic acid biosynthesis pathway were modified to further enhance L-tyrosine production.In addition,the phosphoketolase pathway was introduced in combination with cofactor engineering to redirect carbon flux to the shikimate pathway.Finally,after adaptive laboratory evolution to low pH an optimal strain was obtained.The strain can produce 92.5 g/L of L-tyrosine in a 5-L fermenter in 62 h,with a yield of 0.266 g/g glucose.

关 键 词：L-TYROSINE Cofactor regeneration Phosphoketolase pathway Escherichia coli 

分 类 号：Q93[生物学—微生物学]