The presence of foci of Rickettsia conorii infection in China  

作　　者：Nannan Xu Hui Liu Chunmei Qu Sai Wen Wenlu Zou Caiyun Chang Gang Wang 

机构地区：[1]Department of Infectious Disease,Qilu Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250012,China [2]Institute of Bacterial Disease,Jinan Center for Disease Control and Prevention,Jinan 250021,China [3]Institute for Infectious Disease Control,Jinan Center for Disease Control and Prevention,Jinan 250021,China

出　　处：《Infectious Medicine》2023年第4期334-337,共4页感染医学（英文）

摘　　要：China is not considered as an endemic area of Rickettsia conorii,so there is no routine clinical way to diagnose this infection.This study aims to determine whether 2 febrile patients who had a tick bite in East China were indeed infected with R.conorii.The citrate synthase gene(gltA)was amplified with universal rickettsial primers by real-time fluorescent PCR from the patients’blood samples.Nested PCR was used to amplify the outer membrane protein A gene(ompA)for positive specimens.PCR products were further identified and analyzed through nucleic acid sequencing.Positive amplification of the gltA and ompA genes was found in both patients.The nucleotide sequences(303 bp)of the ompA gene of the 2 patients had high homology(99%)with the R.conorii Indian tick typhus strain in GenBank.A more than 4-fold increase in IgG against R.conorii provided supportive evidence of SFG Rickettsia infection.And the rapid recovery after doxycycline treatment also supported a rickettsial cause for the disease.Physicians in East China should be aware of human infections with R.conorii.PCR-based diagnostic methods offer a rapid and precise way to diagnose rickettsiosis,improving patient identification and management.

关 键 词：Rickettsia conorii Human infection China 

分 类 号：R51[医药卫生—内科学]