Use of eschar swab DNA to diagnose Rickettsia conorii subspecies conorii infection in Crimea:A case report  

作　　者：Emine E.Alieva Muniver T.Gafarova Eugene I.Bondarenko Igor A.Dovgan Irina I.Osiptchuk Marina E.Eremeeva 

机构地区：[1]Federal State Budgetary Institution‘N.I.Pirogov Saki Military Clinical Sanatorium’,Ministry of Defense of Russia,Saki 296500,Russia [2]S.I.Georgievsky Medical Academy(Academic Unit),V.I.Vernadsky Crimean Federal University,Simferopol 295051,Russia [3]Vector-Best,Novosibirsk 630117,Russia [4]Saki Regional Hospital,Saki 296500,Russia [5]Jiann-Ping Hsu College of Public Health,Georgia Southern University,Statesboro,GA 30458,USA

出　　处：《Infectious Medicine》2023年第4期338-342,共5页感染医学（英文）

摘　　要：Mediterranean spotted fever(MSF)has been diagnosed clinically in the Crimean Peninsula since the 1930s.We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever,maculopapular rash,and eschar.Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 Rickettsia protein genes.The strain causing clinical illness was characterized as Rickettsia conorii subspecies conorii Malish 7.This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management.

关 键 词：ESCHAR Mediterranean spotted fever PCR Rickettsia conorii SWAB 

分 类 号：R51[医药卫生—内科学]