A study implementing real-time PCR to identify Strongyloides species of third-stage larvae in human stool samples from Southern Vietnam  

作　　者：Le Duc Vinh Nguyen Kim Thach Huynh Hong Quang Do Nhu Binh Tran Thi Duc Hanh Nguyen Minh Toàn Nguyen Trung Tuyen Nguyen Thu Huong 

机构地区：[1]Department of Medical Parasitology,Pham Ngoc Thach University of Medicine,Ho Chi Minh City 700000,Vietnam [2]Department of Fundamental Chemistry-Biochemistry,Pham Ngoc Thach University of Medicine,Ho Chi Minh City 700000,Vietnam [3]Clinical Epidemiology Research Unit,Institute of Malariology,Parasitology,and Entomology Quy Nhon,Binh Dinh 55000,Vietnam [4]Department of Infectious Disease,Military Hospital 103,Hanoi 100000,Vietnam [5]Department of Epidemiology,Hanoi University of Public Health,Hanoi 100000,Vietnam [6]Department of Microbiology-Parasitology,Hanoi University of Public Health,Hanoi 100000,Vietnam [7]Department of Medicine,University of Medicine and Pharmacy,Vietnam National University,Hanoi,100000 Vietnam

出　　处：《Infectious Medicine》2023年第3期229-236,共8页感染医学（英文）

摘　　要：Background:Strongyloidiasis,a neglected disease caused by intestinal nematodes of the genus,is endemic to tropical and subtropical areas such as Vietnam.Morphological methods only identify the genus,while DNA-molecular techniques are susceptible in Strongyloides spp.detection.The study aims to determine the prevalence of dominant Strongyloides species among the population in Duc Hoa district,Long An,Vietnam.Methods:A cross-sectional study used 1190 stool specimens collected from July 2017 to November 2018.All samples were transported within 2 h,stored at 2-8℃,and processed within 48 h for microscopy smear and culture at the Laboratory of Medical Parasitology,Pham Ngoc Thach University of Medicine(PNT).Then all positive samples with the above 2 methods were verified by real-time PCR technique.Real-time PCR amplification was conducted at the Laboratory of Molecular Biology,PNT.Results:Direct microscopy and modified Harada-Mori culture detected Strongyloides spp.larvae in 79/1190 sam-ples(6.6%).About 94.2%of the DNA samples were Strongyloides stercoralis,2.9%were co-infections with Strongy-loides ratti and S.stercoralis,and 2.9%were patients with S.ratti.The identity of 12/14 sequences was confirmed as S.stercoralis with a high level of similarity(91.3%-100%)and over 98%for S.ratti.Conclusion:DNA-molecular techniques and sequence analysis are highly suitable for identifying Strongyloides species isolated from stool samples.It is remarkable evidence of the presence of zoonosis S.ratti disease in human,not just the known S.stercoralis.It is likely to result in a certain proportion of people being infected by this animal-borne infectious pathogen.

关 键 词：STRONGYLOIDIASIS Strongyloides stercoralis Strongyloides ratti DNA-molecular techniques 

分 类 号：R446.13[医药卫生—诊断学]