Lactobacillus johnsonii N5 from heat stress-resistant pigs improves gut mucosal immunity and barrier in dextran sodium sulfate-induced colitis  

作　　者：Long Yuan Chuyang Zhu Fang Gu Miaonan Zhu Jiacheng Yao Cuipeng Zhu Shicheng Li Kun Wang Ping Hu Yunzeng Zhang Demin Cai Hao-Yu Liu 

机构地区：[1]College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China [2]Joint International Research Laboratory of Agricultural&Agri-Product Safety,The Ministry of Education of China,Yangzhou University,Yangzhou 225009,China [3]Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou University,Yangzhou 225009,China

出　　处：《Animal Nutrition》2023年第4期210-224,共15页动物营养（英文版）

基　　金：supported by the Natural Science Foundation of Jiangsu Province(BK20200932,BK20220582);the National Natural Science Foundation of China(32002243);the Postgraduate Research and Practice Innovation Program of Yangzhou University(X20211025);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).

摘　　要：Developing effective strategies to prevent diarrhea and associated-gut disorders in mammals has gained great significance.Owing to the many health benefits provided by the commensal microbiota of the intestinal tract,such as against environmental perturbation,we explored the host phenotype-associated microbes and their probiotic potential.Based on the observations that the chronic heat stress-exposed weaned piglets present as heat stress-susceptible(HS-SUS)or heat stress-resistant(HS-RES)individuals,we confirmed the phenotypic difference between the two on growth performance(P<0.05),diarrhea index(P<0.001),intestinal heat shock protein 70(HSP70)regulation(P<0.01),and inflammatory responses(P<0.01).By comparing the gut microbiome using 16S rRNA gene sequencing and KEGG functional analysis,we found that Lactobacillus johnsonii exhibited significantly higher relative abundance in the HS-RES piglets than in the HS-SUS ones(P<0.05).Further experiments using a mouse model for chemical-induced inflammation and intestinal injury demonstrated that oral administration of a representative L.johnsonii N5(isolated from the HS-RES piglets)ameliorated the clinical and histological signs of colitis while suppressing intestinal pro-inflammatory cytokines TNF-a and IL-6 production(P<0.05).We found that N5 treatment enhanced tight junction proteins ZO-1 and occludin and cytoprotective HSP70 levels under physiological condition and restored their mucosal expressions in colitis(P<0.05).In support of the high production of the anti-inflammatory cytokine IL-10,N5 promoted the intestinal Peyer’s patches MHCⅡ+and CD103+dendritic cell populations(P<0.05),increased the regulatory T(Treg)cell numbers(P<0.05),and decreased the Th17 population and its IL-17a production under physiological condition and during colitis(P<0.01).Our results shed light on understanding the interaction between commensal Lactobacillus and the host health,and provide L.johnsonii N5 as an alternative to antibiotics for preventing diarrhea and intestinal diseases.

关 键 词：Lactobacillus johnsonii Probiotic Heat shock protein DIARRHEA Heat stress Treg/Th17 

分 类 号：S852.6[农业科学—基础兽医学]